|
| Status |
Public on Oct 18, 2019 |
| Title |
MDA-C, biological replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
Parental MDA-MB-231
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Triple-negative, mesenchymal
|
| Treatment protocol |
Cells were transduced using a lentiviral system to stably express control GFP (MDA231-Control, MDA231-C) and PRRX1 (MDA231-PRRX1, MDA231-P)
|
| Growth protocol |
Cells were cultured in DMEM:F12 HAM media (1:1), and SUM149PT cells in F12 HAM media, supplemented with 10% heat inactivated fetal bovine serum (FBS) (Sigma), 10 μg/ml insulin (Roche), 1% Gentamicin (Sigma) and 1% amphotericin (Sigma).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extractions were performed from cells in 70% confluency in 10cm plates, using miRvana kit following manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Done according to manufacturer's protocol
|
| |
|
| Hybridization protocol |
Three independent samples per condition from MDA231-C and MDA231-P cells, were hybridized to Human Gene 2.0 ST expression arrays (Affymetrix)
|
| Scan protocol |
Done according to manufacturer's protocol
|
| Description |
Gene expression data from MDA231 control cells
|
| Data processing |
Microarray data was analyzed using R and Bioconductor. Expression data was preprocessed and normalized using the RMA algorithm
Differential expression analysis was performed using limma t- test to extract statistically significant changes between PRRX1 and control samples
|
| |
|
| Submission date |
Sep 26, 2019 |
| Last update date |
Oct 18, 2019 |
| Contact name |
Angela Nieto |
| E-mail(s) |
anieto@umh.es
|
| Organization name |
Instituto de Neurociencias (CSIC-UMH)
|
| Department |
Developmental Neurobiology
|
| Lab |
Cell Movements in Development and Disease
|
| Street address |
Av. Ramón y Cajal s/n
|
| City |
San Juan de Alicante |
| State/province |
Alicante |
| ZIP/Postal code |
03550 |
| Country |
Spain |
| |
|
| Platform ID |
GPL16686 |
| Series (1) |
| GSE138078 |
PRRX1 overexpression in MDA-MB-231 breast cancer cells |
|
