|
| Status |
Public on Jul 08, 2020 |
| Title |
Effects of ascorbic acid on topotecan-induced DNA damage in human tumor cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To investigate mechanisms and differential gene expressions in topotecan-induced DNA damage and effects of ascorbic acid/hydrogen peroxide on these genes in topotecan cytotoxicity in tumor cells
|
| |
|
| Overall design |
MCF-7 breast tumor cells were treated with toptecan and combinations of topotecan and ascorbic acid in RPMI 1640 media containing 10% FBS for various times. Cells wre lysed with Triazol reagent and tyotal RNA was extracted and purified.
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| |
|
| Contributor(s) |
Sinha BK |
| Citation(s) |
32765594 |
| |
| Submission date |
Oct 04, 2019 |
| Last update date |
Aug 10, 2020 |
| Contact name |
NIEHS Microarray Core |
| E-mail(s) |
microarray@niehs.nih.gov, liuliw@niehs.nih.gov
|
| Organization name |
NIEHS
|
| Department |
DIR
|
| Lab |
Microarray Core
|
| Street address |
111 T.W. Alexander Drive
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
|
| Samples (24)
|
|
| Relations |
| BioProject |
PRJNA575871 |
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