|
| Status |
Public on Jul 08, 2020 |
| Title |
1130_Asc_Top Rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
MCF-7, ascorbic acid + topotecan, 0 hour, rep 3
|
| Organism |
Homo sapiens |
| Characteristics |
cells: MCF-7
gender: Female
|
| Treatment protocol |
Cell were left untreated or treated with ascorbic acid , topotecan or combinations of ascorbic acid and topotecan for 1 hr and 24 hr.
|
| Growth protocol |
MCF-7 breast tumor cells were grown in RPMI 1640 media containing 10% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Triazol method.
|
| Label |
Cy3
|
| Label protocol |
Starting with 500ng of total RNA, Cy3 labeled cRNA was produced per manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
For each sample, 1.65ug of Cy3 labeled cRNAs were fragmented and hybridized for 17 hours in a rotating hybridization oven.
|
| Scan protocol |
Slides were washed and then scanned with an Agilent Scanner.
|
| Description |
Gene expression from MCF-7, ascorbic acid + topotecan, 0 hour
|
| Data processing |
Data was obtained using the Agilent Feature Extraction software (v12), using the 1-color defaults for all parameters.
|
| |
|
| Submission date |
Oct 04, 2019 |
| Last update date |
Jul 08, 2020 |
| Contact name |
NIEHS Microarray Core |
| E-mail(s) |
microarray@niehs.nih.gov, liuliw@niehs.nih.gov
|
| Organization name |
NIEHS
|
| Department |
DIR
|
| Lab |
Microarray Core
|
| Street address |
111 T.W. Alexander Drive
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL4133 |
| Series (1) |
| GSE138442 |
Effects of ascorbic acid on topotecan-induced DNA damage in human tumor cells |
|
