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| Status |
Public on Sep 21, 2022 |
| Title |
Gene expression of mouse oral cancer cell lines |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We established two murine OSCC cell lines, named M1-2 and M2-3, from a mouse tumor induced by 4-nitroquinoline 1-oxide (4-NQO)/arecoline.
After in vitro selection using sphere culture, NHRI-HN1 and NHRI-HN2 were derived from M1-2 and M2-3 cells, respectively. Only NHRI-HN1 cells are capable of generating orthotopic tumors in syngeneic mice.
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| Overall design |
Total RNA was extracted using Trizol reagent and purified using RNeasy mini kit. Genome-wide gene expression analysis was performed using the Affymetrix Mouse Clariom S Assay. Microarray analysis were conducted as followed the standard Affymetrix procedures. Hybridized slides were scanned by Affymetrix Gene Chip scanner 3000 7G system. Raw data were generated using Partek Genomics Suite version 7 software.
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| Web link |
https://pubmed.ncbi.nlm.nih.gov/31878324/
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| |
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| Contributor(s) |
Chen Y, Chen Y |
| Citation(s) |
31878324 |
| |
| Submission date |
Oct 23, 2019 |
| Last update date |
Sep 22, 2022 |
| Contact name |
Ya-Wen Chen |
| E-mail(s) |
ywc@nhri.org.tw
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| Organization name |
National Health Research Institutes
|
| Department |
National Institute of Cancer Research
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| Street address |
35, Keyan Rd, Zhunan Town
|
| City |
Miaoli County |
| ZIP/Postal code |
35053 |
| Country |
Taiwan |
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| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA579101 |
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