|
| Status |
Public on Sep 21, 2022 |
| Title |
NHRI-HN2 |
| Sample type |
RNA |
| |
|
| Source name |
Derivation from M2-3 cells by sphere culture
|
| Organism |
Mus musculus |
| Characteristics |
cell type: oral cancer cell line
|
| Growth protocol |
Cells were stably cultured in dishes with DMEM with 10% fetal bovine serum at 37°C in an incubator supplied with 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol reagent and purified using RNeasy mini kit.
|
| Label |
biotin
|
| Label protocol |
Genome-wide gene expression analysis was performed using the Affymetrix Mouse Clariom S Assay.
|
| |
|
| Hybridization protocol |
Microarray analysis were conducted as followed the standard Affymetrix procedures.
|
| Scan protocol |
Hybridized slides were scanned by Affymetrix Gene Chip scanner 3000 7G system.
|
| Description |
Gene expression data from total RNA of mouse cell lines
|
| Data processing |
Raw data were generated using Partek Genomics Suite version 7 software.
|
| |
|
| Submission date |
Oct 23, 2019 |
| Last update date |
Sep 21, 2022 |
| Contact name |
Ya-Wen Chen |
| E-mail(s) |
ywc@nhri.org.tw
|
| Organization name |
National Health Research Institutes
|
| Department |
National Institute of Cancer Research
|
| Street address |
35, Keyan Rd, Zhunan Town
|
| City |
Miaoli County |
| ZIP/Postal code |
35053 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL23038 |
| Series (1) |
| GSE139282 |
Gene expression of mouse oral cancer cell lines |
|
