GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE139873 Query DataSets for GSE139873
Status Public on Nov 05, 2019
Title Expression analysis of Caulobacter crescentus NA1000 bearing a CCNA_00690 (cdnL) deletion [dcdnL] vs Caulobacter crescentus NA1000 wild type [wt]
Organism Caulobacter vibrioides NA1000
Experiment type Expression profiling by array
Summary Investigation of whole genome gene expression level changes in a Caulobacter crescentus NA1000 dcdnL mutant, compared to the wild-type strain.
In bacteria, transcription of housekeeping genes required for metabolic homeostasis and cell proliferation is guided by the sigma factor σ70. The conserved CarD-like transcriptional regulator, CdnL, associates with promoter regions where σ70 localizes and stabilizes the open promoter complex. Caulobacter crescentus cells lacking CdnL have severe morphological and growth defects. Our microarray experiment demonstrates how cdnL deletion affects the transcriptome of Caulobacter crescentus.
Overall design A six chip study using total RNA recovered from three separate wild-type cultures of Caulobacter crescentus NA1000 and three separate cultures of a mutant strain, Caulobacter crescentus NA1000 dcdnL (deletion of CCNA_00690), in which the cdnL gene coding for an RNA polymerase associated factor is deleted. Each chip measures the expression level of 3933 genes from Caulobacter crescentus NA1000 with 3 probes per gene and with three-fold technical redundancy.
Contributor(s) Collier-Close J, Gonzalez D
Citation(s) 31961855
Submission date Nov 04, 2019
Last update date Feb 04, 2020
Contact name Diego Gonzalez
Organization name University of Lausanne
Department Department of Fundamental Microbiology
Lab Prof. Justine Collier
Street address Quartier UNIL-Sorge, Bâtiment Biophore
City Lausanne
ZIP/Postal code 1015
Country Switzerland
Platforms (1)
GPL17931 110420_Ccre_JC_exp, 4plex array
Samples (6)
GSM4148030 Ccrescentus_wt_rep1
GSM4148031 Ccrescentus_wt_rep2
GSM4148032 Ccrescentus_wt_rep3
BioProject PRJNA587418

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE139873_RAW.tar 12.0 Mb (http)(custom) TAR (of CALLS, PAIR)
Processed data included within Sample table
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap