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| Status |
Public on Apr 01, 2020 |
| Title |
Capability of FDA-EVIR Microarray for Detection of Norovirus and Hepatitis A Virus in Inoculated Tomatoes, Green Onions, and Celery |
| Platform organisms |
Bacillus subtilis; unidentified adenovirus; Rotavirus; Coxsackievirus; Hepatovirus A; Sapovirus; Norovirus; Astrovirus sp.; Paslahepevirus balayani |
| Sample organisms |
Apium graveolens; Solanum lycopersicum; Allium fistulosum |
| Experiment type |
Expression profiling by genome tiling array
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| Summary |
The capability of the U.S. Food and Drug Administration Enteric Viruses tiling microarray (FDA-EVIR) was assessed for rapid molecular identification of human norovirus (NoV) and hepatitis A virus (HAV) extracted from artificially inoculated fresh produce. Two published viral extraction strategies, total RNA extraction or virus particle isolation, were employed to prepare the viral targets. We also assessed the amount of viral RNA extracted from celery by three commercially-available kits and how well that RNA performed on the FDA-EVIR. Our results confirm that FDA-EVIR can correctly identify common enteric viruses isolated from fresh produce and is capable of identifying single and mixed species of viruses, as well as distinguishing among genotypes. Extending microarray methods to other food matrices should provide important support to surveillance and outbreak investigations.
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| Overall design |
In total 17 samples; 11 Input files (different produces/viruses/extraction methods) with the corresponding 6 negative control samples
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| Contributor(s) |
Yu C, Hida K, Papafragkou E |
| Citation(s) |
32379890 |
| |
| Submission date |
Nov 13, 2019 |
| Last update date |
Jul 01, 2020 |
| Contact name |
Christine Fang Yu |
| E-mail(s) |
Christine.Yu@fda.hhs.gov
|
| Phone |
1-301-796-0643
|
| Organization name |
US Food and Drug Administration
|
| Department |
Center for Food Safety and Applied Nutrition
|
| Lab |
Office of Applied Research and Safety Assessment
|
| Street address |
8301 Muirkirk Rd.
|
| City |
Laurel |
| State/province |
MD |
| ZIP/Postal code |
20708 |
| Country |
USA |
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| Platforms (1) |
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| Samples (17)
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| Relations |
| BioProject |
PRJNA589332 |
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