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| Status |
Public on Feb 22, 2021 |
| Title |
Characterization of Interleukin 11-producing fibroblasts that constitute a niche for tumor development [Agilent] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Interleukin (IL)-11 belongs to a member of the IL-6 family of cytokines and is involved in a variety of cellular responses. However, the origin and functions of IL-11-producing (IL-11+) cells are not fully understood. To characterize IL-11+ cells in vivo, we generated Il11 reporter mice. IL-11+ cells rapidly appeared in the colon of mice after dextran sulfate sodium treatment. IL-11+ cells expressed fibroblast markers and genes associated with cell proliferation and tissue repair. IL-11+ cells also appeared in the colon of three murine tumor models and human colorectal cancers. Deletion of Il11ra1 or Il11 attenuated the development of colitis-associated colorectal cancers in mice. IL-11 induced STAT3 phosphorylation in colonic fibroblasts, and injection of IL-11 receptor agonist upregulated many genes that were enriched in IL-11+ cells. Together, tumor cells induced IL-11+ fibroblasts, and a feed-forward loop between IL-11 and IL-11+ fibroblasts might constitute a niche for promoting tumor development.
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| Overall design |
To identify target genes by IL-11 in the colon, we intravenously injected 10 mg of the IL-11 receptor agonist, NT-3N into wild-type C57BL/B6 mice (n=2-3 mice per each time point). Then mice were sacrificed, and the colon was removed at 3 hours after injection.
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| Contributor(s) |
Nishina T, Nakano H |
| Citation(s) |
33863879 |
| Submission date |
Feb 19, 2021 |
| Last update date |
May 13, 2021 |
| Contact name |
Takashi Nishina |
| E-mail(s) |
takashi.nishina@med.toho-u.ac.jp
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| Phone |
+81-3-3762-4151
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| Organization name |
Toho University
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| Department |
Biochemistry
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| Street address |
5-21-16 Omori-nishi
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| City |
Ota-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
143-8540 |
| Country |
Japan |
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| Platforms (1) |
| GPL21163 |
Agilent-074809 SurePrint G3 Mouse GE v2 8x60K Microarray [Probe Name version] |
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| Samples (5)
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| This SubSeries is part of SuperSeries: |
| GSE141644 |
Characterization of Interleukin 11-producing fibroblasts that constitute a niche for tumor development |
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| Relations |
| BioProject |
PRJNA703022 |
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