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| Status |
Public on Dec 27, 2019 |
| Title |
New insights into the Manila clam – Perkinsus olseni interaction based on gene expression analysis of clam hemocytes and parasite trophozoites through in vitro challenges [Perkinsus olseni] |
| Organism |
Perkinsus olseni |
| Experiment type |
Expression profiling by array
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| Summary |
The Manila clam (Ruditapes philippinarum) is the bivalve species with the highest world production from both fisheries and aquaculture, but its production is seriously threatened by perkinsosis, a disease caused by the protozoan parasite Perkinsus olseni. To understand the molecular mechanisms underlying R. philippinarum–P. olseni interaction, we analyzed the gene expression profiles of in vitro challenged clam hemocytes and P. olseni trophozoites, using two oligo-microarray platforms, one previously validated for R. philippinarum hemocytes and a new one developed and validated in this study for P. olseni. Manila clam hemocytes were in vitro challenged with trophozoites, zoospores, and extracellular products from P. olseni in vitro cultures, while P. olseni trophozoites were in vitro challenged with Manila clam plasma along the same time-series (1 h, 8 h, and 24 h). The hemocytes showed a fast activation of the innate immune response, particularly associated with hemocyte recruitment, in the three types of challenges. Nevertheless, different immune-related pathways were activated in response to the different parasite stages, suggesting specific recognition mechanisms. Furthermore, the analyses provided useful complementary data to previous in vivo challenges, and confirmed the potential of some proposed biomarkers. The combined analysis of gene expression in host and parasite identified several processes in both the clam and P. olseni, such as redox and glucose metabolism, protease activity, apoptosis and iron metabolism, whose modulation suggests cross-talk between parasite and host. This information might be critical to determine the outcome of the infection, thus highlighting potential therapeutic targets. Altogether, the results of this study aid to understand the response and interaction between R. philippinarum–P. olseni and will contribute for developing effective control strategies for this threatening parasitosis.
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| Overall design |
P. olseni trophozoites (~ 5x10e6) resuspended in 2.5 mL filtered seawater (FSW) were placed in IWAKI 6-well plates, and 2.5 mL of plasma (treatment) or FSW (control) were added into a permeable insert (0.2 µm Anopore® membrane NUNC 25 mm) set in the plate-wells. Four or three pseudo-replicates (trophozoites from the same culture) for both control and treatment groups were collected at 1, 8 and 24 h since the onset of the challenge.
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| Contributor(s) |
Hasanuzzaman AF, Cao A, Ronza P, Fernández-Boo S, Rubiolo JA, Robledo D, Gómez-Tato A, Alvarez-Dios JA, Pardo BG, Villalba A, Martínez P |
| Citation(s) |
32087247 |
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| Submission date |
Dec 27, 2019 |
| Last update date |
Mar 29, 2020 |
| Contact name |
Belen G Pardo |
| E-mail(s) |
belen.gomez@usc.es
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| Phone |
+34 982822428
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| Organization name |
University of Santiago de Compostela
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| Department |
Zoology, Genetics and Physical Anthropology
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| Lab |
Acuigen
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| Street address |
Avda. Carballo Calero s/n
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| City |
LUGO |
| State/province |
LUGO |
| ZIP/Postal code |
27002 |
| Country |
Spain |
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| Platforms (1) |
| GPL27953 |
[Agilent-068823] Agilent USC-Acuigen Perkinsus olseni 15 K v 1.0 ID-068823 |
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| Samples (16)
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| This SubSeries is part of SuperSeries: |
| GSE142666 |
New insights into the Manila clam – Perkinsus olseni interaction based on gene expression analysis of clam hemocytes and parasite trophozoites through in vitro challenges |
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| Relations |
| BioProject |
PRJNA597889 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE142665_RAW.tar |
34.6 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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