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| Status |
Public on Jul 06, 2021 |
| Title |
Polymerase II binding analysis during nutrient restriction using targeted DamID |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The adaptable transcriptional response to changes in food availability not only ensures animal survival, but also permits progressing with embryonic development. Interestingly, the central nervous system is preferentially protected to periods of malnutrition, a phenomenon known as ‘brain sparing’. However, the mechanisms that mediates this genetic response remains poorly understood. To get a better understanding of this, we used Drosophila melanogaster as a genetic model, analysing the transcriptional response of larval neural stem cells (neuroblasts) and glial cells of the blood-brain barrier during nutrient restriction using the targeted DamID technique.
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| Overall design |
Polymerase II Targeted DamID using neuroblasts and blood-brain barrier glial drivers. Drosophila larvae were raised for 8 days at 18°C and transferred to 29°C in nutrient restriction or normal fed conditions.
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| Contributor(s) |
Contreras EG |
| Citation(s) |
34210781 |
| |
| Submission date |
Feb 10, 2020 |
| Last update date |
Jul 13, 2021 |
| Contact name |
Esteban Gonzalo Contreras Sepúlveda |
| E-mail(s) |
eocontre@uc.cl
|
| Organization name |
Universidad de Chile
|
| Street address |
Independencia 1027
|
| City |
Santiago |
| ZIP/Postal code |
8380453 |
| Country |
Chile |
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| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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| Samples (36)
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| Relations |
| BioProject |
PRJNA605807 |
| SRA |
SRP248009 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE145055_mdr65_Pol.Fed1.bedgraph.gz |
5.0 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE145055_mdr65_Pol.Fed2.bedgraph.gz |
5.0 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE145055_mdr65_Pol.Fed3.bedgraph.gz |
4.9 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE145055_mdr65_Pol.Fed4.bedgraph.gz |
4.9 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE145055_mdr65_Pol.Fed5.bedgraph.gz |
4.9 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE145055_mdr65_Pol.NR1.bedgraph.gz |
4.9 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE145055_mdr65_Pol.NR2.bedgraph.gz |
4.9 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE145055_mdr65_Pol.NR3.bedgraph.gz |
4.9 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE145055_sema5c_Pol.Fed1.bedgraph.gz |
4.8 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE145055_sema5c_Pol.Fed2.bedgraph.gz |
4.8 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE145055_sema5c_Pol.Fed3.bedgraph.gz |
4.7 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE145055_sema5c_Pol.NR1.bedgraph.gz |
4.8 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE145055_sema5c_Pol.NR2.bedgraph.gz |
4.9 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE145055_sema5c_Pol.NR3.bedgraph.gz |
4.9 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE145055_wor_Pol.Fed1.bedgraph.gz |
4.5 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE145055_wor_Pol.Fed2.bedgraph.gz |
4.9 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE145055_wor_Pol.NR1.bedgraph.gz |
4.8 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE145055_wor_Pol.NR2.bedgraph.gz |
4.9 Mb |
(ftp)(http) |
BEDGRAPH |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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