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| Status |
Public on Aug 03, 2009 |
| Title |
GSK3 activates telomerase by regulating dynamic behaviour of multiple transcription factors converging on the hTERT gene |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Telomerase controls cell immortality and is a promising cancer target. Understanding transcriptional regulation of the catalytic subunit hTERT is critical to realise effective telomerase therapeutics. GSK3 inhibition suppresses hTERT expression.
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| |
|
| Overall design |
A2780 ovarian adenocarcinoma cells were cultured continuously for 21 days in the presence of DMSO or 2.5mM GSK3 inhibitor 6-bromoindirubin-3’-oxime (BIO) with replenishment weekly on days 1 and 4. Cells were subcultured, counted, and RNA samples taken.
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| |
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| Contributor(s) |
Bilsland AE, Hoare S, Stevenson K, Plumb J, Gomez-Roman N, Cairney C, Burns S, Lafferty-Whyte K, Roffey J, Hammonds T, Keith WN |
| Citation(s) |
19649288 |
| |
| Submission date |
Jan 23, 2009 |
| Last update date |
Jan 23, 2019 |
| Contact name |
W. Nicol Keith |
| E-mail(s) |
n.keith@beatson.gla.ac.uk
|
| Organization name |
The University of Glasgow
|
| Street address |
Center for Oncology and Applied Pharmacology, University of Glasgow, CRUK Beatson Laboratories, Garscube Estate, Switchback Road
|
| City |
Glasgow |
| State/province |
Lanarkshire |
| ZIP/Postal code |
G61 1BD |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
|
| Samples (6)
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| Relations |
| BioProject |
PRJNA111611 |
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