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| Status |
Public on Apr 27, 2020 |
| Title |
Identification of genes regulated by Foxc1, which is a host target of miR-138 |
| Organisms |
Mus musculus; Human alphaherpesvirus 1 |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In other parts of this study we identified Foxc1 as a host target of miR-138 and found that Foxc1 can promote herpes simplex virus-1 (HSV-1) replication. To understand the mechanisms of this function, we performed an RNAseq experiment comparing vector (pcDNA) and pFoxchuman transfected Neuro-2a cells. After transfection, these cells were infected with HSV-1 for 5 hours at an MOI of 1 to identify viral genes regulated by Foxc1 as well as host genes.
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| Overall design |
We transfected Neuro-2a cells with pFoxc1human for 40 hours and then infected with KOS for 5 hours at an MOI of 1 before RNAseq analysis of these cells. This dataset contain three replicates for pcDNA transfected cells and three replicates for pFoxc1human transfected cells.
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| Contributor(s) |
Pan D, Coen DM |
| Citation(s) |
33558653 |
| |
| Submission date |
Feb 18, 2020 |
| Last update date |
Mar 16, 2021 |
| Contact name |
Dongli Pan |
| E-mail(s) |
pandongli@zju.edu.cn
|
| Organization name |
Zhejiang University
|
| Street address |
866 Yuhangtang Road
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| City |
Hangzhou |
| State/province |
Zhejiang |
| ZIP/Postal code |
110058 |
| Country |
China |
| |
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| Platforms (1) |
| GPL28172 |
BGISEQ-500 (Human alphaherpesvirus 1; Mus musculus) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE127504 |
Identification of herpes simplex virus 1 and host targets of miR-138 by combined RNAseq/PARCLIP |
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| Relations |
| BioProject |
PRJNA607320 |
| SRA |
SRP249911 |
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