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| Status |
Public on Oct 20, 2020 |
| Title |
Whole Transcriptome analysis for porcine alveolar macrophage infected by African Swine Fever Virus |
| Organism |
Sus scrofa |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
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| Summary |
African swine fever virus (ASFV) is one of the most devastating swine pathogens characterized by nearly 100% mortality in naive herds and was recently emerged the in China. In this study, we generated the expression profile of porcine alveolar macrophages (PAMs) infected with a high pathogenic ASFV (Pig/Heilongjiang/2018 (Pig/HLJ/18) ASFV). Our data indicated that ASFV infection lead to a strong inhibition of host immunity but promote chemokine-mediated signaling pathway and neutrophil chemotaxis. Moreover, ASFV infection can modulate the host miRNA involved regulation network, leading to a significant increase of host metabolism related genes and acceleration of virus replication. Furthermore, ASFV-derived viral small RNAs (vsRNAs) can target some host immune response related genes. In conclusion, our transcriptome-wide data provide some insights into the regulatory mechanism during ASFV infection.
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| Overall design |
MicroRNA and mRNA profiles of porcine alveolar macrophages (PAMs) at different time-points post ASFV infection. MicroRNA and mRNA datasets were generated by deep sequencing, in triplicate, using Illumina Nextseq CN500 and Hiseq Xten platforms, respectively.
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| Contributor(s) |
Ding Q, Ju X, Li J, Nan Y |
| Citation(s) |
34481273 |
| |
| Submission date |
Feb 26, 2020 |
| Last update date |
Apr 03, 2023 |
| Contact name |
Jingrui Li |
| E-mail(s) |
jrli@cau.edu.cn
|
| Organization name |
China Agricultural Universtiy
|
| Street address |
Yuanmingyuan West Road No.2
|
| City |
Beijing |
| ZIP/Postal code |
100193 |
| Country |
China |
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| Platforms (2) |
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| Samples (42)
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| Relations |
| BioProject |
PRJNA608868 |
| SRA |
SRP250794 |
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