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| Status |
Public on Feb 28, 2020 |
| Title |
Rewiring of gene expression in PWBC at artificial insemination is predictive of pregnancy outcome in cattle (Bos taurus) |
| Organism |
Bos taurus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Infertility is a disease that affects humans and cattle in similar ways. The resemblance includes complex genetic architecture, multiple etiology, low heritability of fertility related traits in females, and the frequency in the female population. Here, we used cattle as a biomedical model to test the hypothesis that gene expression profiles of protein-coding genes expressed in peripheral white blood cells (PWBCs), and circulating micro RNAs in plasma, are associated with female fertility, measured by pregnancy outcome. We drew blood samples from 17 female calves on the day of artificial insemination and analyzed transcript abundance for 10496 genes in PWBCs and 290 circulating micro RNAs. The females were later classified as pregnant to artificial insemination, pregnant to natural breeding or not pregnant. We identified 1860 genes producing significant differential coexpression (eFDR<0.002) based on pregnancy outcome. Additionally, 237 micro RNAs and 2274 genes in PWBCs presented differential coexpression based on pregnancy outcome. Furthermore, using a machine learning prediction algorithm we detected a subset of genes whose abundance could be used for blind categorization of pregnancy outcome. Our results provide strong evidence that bloodborne transcript abundance is highly associated with fertility in females.
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| Overall design |
17 heifers classified as not-pregnant (n=5), AI-pregnant (n=6) and NB-pregnant (n=6)
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| Contributor(s) |
Biase F, Moorey S |
| Citation(s) |
33033295 |
| Submission date |
Feb 27, 2020 |
| Last update date |
Nov 02, 2021 |
| Contact name |
Fernando H Biase |
| E-mail(s) |
fbiase@vt.edu
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| Phone |
540-231-9520
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| Organization name |
Virginia Tech
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| Department |
School of Animal Sciences
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| Lab |
Biase lab
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| Street address |
175 West Campus Drive
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| City |
Blacksburg |
| State/province |
VA |
| ZIP/Postal code |
24061 |
| Country |
USA |
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| Platforms (2) |
| GPL23055 |
Illumina NextSeq 500 (Bos taurus) |
| GPL26012 |
Illumina NovaSeq 6000 (Bos taurus) |
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| Samples (34)
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| Relations |
| BioProject |
PRJNA609112 |
| SRA |
SRP250956 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE146041_2020_02_26_unfiltered_count_miRNA_plasma.txt.gz |
216.3 Kb |
(ftp)(http) |
TXT |
| GSE146041_2020_02_26_unfiltered_count_pwbc.txt.gz |
550.2 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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