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| Status |
Public on Apr 17, 2009 |
| Title |
Expression data from ERG Si treated and Control HUVEC cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
ERG (Ets Related Gene) is an ETS transcription factor that was originally described for its role in a number of human cancers. Our preliminary data demonstrate that ERG exhibits a highly EC restricted pattern of expression in cultured primary cells and several adult tissues including the heart, lung, and brain. In response to inflammatory stimuli, such as TNF-alpha, we observed a marked reduction of ERG expression in EC.
To further define the role of ERG in the regulation of normal EC function we used RNA interference to knockdown ERG. Knockdown of ERG in human umbilical vein EC (HUVEC) using siRNA was associated with the reduction of a number known ERG targets.
Keywords: SIRNA Functional Role
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| Overall design |
The functional role of the ERG in the EC cellls was elucidated by comparing gene expression data from HUVEC cells trasfected with only vector (BERG) , non-sense SiRNA (CERG) vs ERG SiRNA (ERG).
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| Contributor(s) |
Yuan L, Nikolova-Krstevski V, Zhan Y, Kondo M, Bhasin M, Varghese L, Carman C, Oettgen P |
| Citation(s) |
19359602 |
| |
| Submission date |
Feb 11, 2009 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Peter Oettgen |
| E-mail(s) |
joettgen@caregroup.harvard.edu
|
| Phone |
617-667-3390
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| Fax |
617-975-5299
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| Organization name |
Beth Israel Deaconess Medical Center
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| Department |
Department of Medicine
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| Lab |
Division of Cardiology
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| Street address |
Harvard Institutes of Medicine, 4 Blackfan Circle
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (6)
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| GSM370399 |
HUVEC treated with ERG SiRNA , biological rep1 |
| GSM370400 |
HUVEC treated with ERG SiRNA , biological rep2 |
| GSM370401 |
HUVEC treated with Non-sense SiRNA , biological rep1 |
| GSM370402 |
HUVEC treated with Non-sense SiRNA biological rep2 |
| GSM370403 |
HUVEC infected with Control Vector , biological rep1 |
| GSM370404 |
HUVEC infected with Control Vector, biological rep2 |
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| Relations |
| BioProject |
PRJNA111971 |
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