|
| Status |
Public on Apr 17, 2009 |
| Title |
HUVEC treated with ERG SiRNA , biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
cell lines HUVEC (human umbilical vein EC)
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HUVEC
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
standard Affymetrix protocole
|
| Scan protocol |
GeneChips were scanned using the Affymetrix standrad scanner..
|
| Description |
HUVEC treated with ERG SiRNA
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Feb 11, 2009 |
| Last update date |
Apr 17, 2009 |
| Contact name |
Peter Oettgen |
| E-mail(s) |
joettgen@caregroup.harvard.edu
|
| Phone |
617-667-3390
|
| Fax |
617-975-5299
|
| Organization name |
Beth Israel Deaconess Medical Center
|
| Department |
Department of Medicine
|
| Lab |
Division of Cardiology
|
| Street address |
Harvard Institutes of Medicine, 4 Blackfan Circle
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE14801 |
Expression data from ERG Si treated and Control HUVEC cells |
|
