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| Status |
Public on Feb 08, 2022 |
| Title |
Transcriptome reprogramming of key transcription factor deletion strains of Saccharomyces cerevisiae compared with the wild type strain grown at normal temperature by RNA-Seq |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Saccharomyces cerevisiae cells have evolved remarkably sophisticated and flexible transcriptional regulatory networks (TRNs) that allow them to survive and thrive in stress conditions, such as high temperature, osmotic and oxidative conditions, etc. Furthermore, transcription factor plays a central role in transcriptional regulatory networks of stress response. To achieve a thorough understanding of master transcription factors and transcriptional regulatory networks in specific response to prolonged thermal stress, we previously sequenced mRNA from the cultures of the wild type strain ScY01a as well as three key transcription factor deletion strains including ScY01a (srb2∆), ScY01a (sin3∆) and ScY01a (mig1∆) grown at 40ºC in biological duplicates. Here, we further sequenced the corresponding samples cultured at 30ºC as a control. Differences in gene expression comparing the transcription factor deletion strains with the wild type strain by RNA deep sequencing revealed a hierarchical transcriptional regulatory network centered on these three transcription factors in S. cerevisiae.
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| Overall design |
mRNA levels in the wild type strain of Saccharomyces cerevisiae ScY01a as well as three key transcription factor deletion strains including ScY01a (srb2∆), ScY01a (sin3∆) and ScY01a (mig1∆) were examined by RNA Sequencing, in duplicate, using on Illumina platform using 150-bp paired-end sequencing. We sequenced 2 samples from ScY01a, 2 samples from ScY01a (srb2∆), 2 samples from ScY01a (sin3∆) and 2 samples from ScY01a (mig1∆), and identified differential expressions in the transcription factor deletion strain versus the wild type strain by using mRNA levels the wild type strain as a reference.
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| Contributor(s) |
Lin Y, Guo Y, Qi X, Gan Y, Wang Q |
| Citation(s) |
35118059 |
| Submission date |
Apr 07, 2020 |
| Last update date |
Feb 08, 2022 |
| Contact name |
Qinhong Wang |
| E-mail(s) |
wang_qh@tib.cas.cn
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| Organization name |
Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
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| Lab |
Wang lab
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| Street address |
32 West 7th Avenue, Tianjin Airport Economic Area
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| City |
Tianjin |
| ZIP/Postal code |
300308 |
| Country |
China |
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| Platforms (1) |
| GPL27812 |
Illumina NovaSeq 6000 (Saccharomyces cerevisiae) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA623520 |
| SRA |
SRP255577 |
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