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| Status |
Public on Feb 19, 2009 |
| Title |
MicroRNAs in the miR-106b family regulate p21/CDKN1A and promote cell cycle progression. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
microRNAs in the miR-106b family are overexpressed in multiple tumor types and are correlated with the expression of genes that regulate the cell cycle. Consistent with these observations, miR-106b family gain of function promotes cell cycle progression, whereas loss of function reverses this phenotype. Microarray profiling uncovers multiple targets of the family, including the cyclin-dependent kinase inhibitor p21/CDKN1A. We show that p21 is a direct target of miR-106b and that its silencing plays a key role in miR-106b-induced cell cycle phenotypes. We also show that miR-106b overrides a doxorubicin-induced DNA damage checkpoint. Thus, miR-106b family members contribute to tumor cell proliferation in part by regulating cell cycle progression and by modulating checkpoint functions.
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| Overall design |
HCT116 Dicerex5 cells were transfected with microRNAs in six-well plates, and RNA was isolated 10 h after transfection. Transcripts containing the miR-106b family hexamers in their 3' UTRs were identified. By microarray analysis, 103 transcripts that contained miR-106b family complementary hexamers in their 3' UTRs were down-regulated by miR-106b, miR-106a, miR-20b, and miR-17-5p within 10 h of transfection.
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| Contributor(s) |
Ivanovska I, Ball AS, Diaz RL, Magnus JF, Kibukawa M, Schelter JM, Kobayashi SV, Lim L, Burchard J, Jackson AL, Linsley PS, CLeary MA |
| Citation(s) |
18212054 |
| Submission date |
Feb 13, 2009 |
| Last update date |
Sep 21, 2012 |
| Contact name |
Irena Ivanovska |
| E-mail(s) |
irena_ivanovska@merck.com
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| Phone |
206-802-7364
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| Organization name |
Merck
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| Street address |
401 Terry Ave N
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| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
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| Platforms (1) |
| GPL4372 |
Rosetta/Merck Human 44k 1.1 microarray |
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| Samples (6)
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| GSM371206 |
HCT116 Dicer-/- #2, mock vs. HCT116 Dicer-/- #2, luc, 25nM, 10hr |
| GSM371207 |
HCT116 Dicer-/- #2, mock vs. HCT116 Dicer-/- #2 miR-17-5p, 25nM, 10hr |
| GSM371208 |
HCT116 Dicer-/- #2, mock vs. HCT116 Dicer-/- #2 miR-20, 25nM, 10hr |
| GSM371209 |
HCT116 Dicer-/- #2, mock vs. HCT116 Dicer-/- #2 miR-106b, 25nM, 10hr |
| GSM371210 |
HCT116 Dicer-/- #2, mock vs. HCT116 Dicer-/- #2 miR-106a, 25nM, 10hr |
| GSM419902 |
HCT116 Dicer-/- #2, mock vs. HCT116 Dicer-/- #2 miR-17-3p, 25nM, 10hr |
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| Relations |
| BioProject |
PRJNA112071 |
| Supplementary data files not provided |
| Processed data included within Sample table |
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