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Series GSE14886 Query DataSets for GSE14886
Status Public on Feb 19, 2009
Title Expression data in HTETOP cells following tetracycline or dexrazoxane treatment
Organism Homo sapiens
Experiment type Expression profiling by array
Summary HTETOP cells, derived from the human fibrosarcoma cell line HT1080, express human topoisomearse II α (TOP2A) exclusively from a tetracycline (TET)-regulated transgene, we used HTETOP cells to differentiate between TOP2A-dependent and –independent apoptotic effects of doxorubicin and dexrazoxane.
We used microarrays to detect global transcriptional changes in HTETOP cells following tetracycline doxycycline or dexrazoxane treatment.

Keywords: Drug treatment comparison
 
Overall design HTETOP cells were treated with 1 µg/ml doxycycline to inhibit TOP2A expression or vehicle for 24 hours, doxycycline-induced trascriptional changes were identified by comparison of the two groups.

HTETOP cells were treated with 100 µM dexrazoxane to inhibit TOP2A activity or vehicle for 24 hours, global trascriptional changes were identified by comparison of the two groups.
 
Contributor(s) Deng S
Citation(s) 19417146
Submission date Feb 18, 2009
Last update date Mar 25, 2019
Contact name Shiwei Deng
E-mail(s) deng@uni-mainz.de
Organization name Uni Mainz
Street address Obere Zahlbacher Str. 67
City Mainz
ZIP/Postal code 55101
Country Germany
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (8)
GSM372018 doxycycline control
GSM372019 doxycycline
GSM373559 dexrazoxane control 1
Relations
BioProject PRJNA111951

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE14886_RAW.tar 63.6 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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