|
| Status |
Public on Feb 24, 2009 |
| Title |
dexrazoxane control 3 |
| Sample type |
RNA |
| |
|
| Source name |
control HTETOP cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HTETOP
|
| Treatment protocol |
HTETOP cells were treated with 100 µM dexrazoxane or vehicle for 24 hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using peqGOLD Total RNA Kit according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized to Human Genome U133 Plus 2.0 GeneChips from Affymetrix.
|
| Scan protocol |
GeneChips were scanned with the GeneChip Scanner 3000.
|
| Description |
>99.5% TOP2A expression can be silenced in HTETOP cells by the addition of tetracycline
pooled RNA from three cell passages was used for hybridization
|
| Data processing |
The images were processed by GeneChip Operating Software 1.3 and the raw data were normalized by Robust Multiarray Analysis (RMA).
|
| |
|
| Submission date |
Feb 23, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Shiwei Deng |
| E-mail(s) |
deng@uni-mainz.de
|
| Organization name |
Uni Mainz
|
| Street address |
Obere Zahlbacher Str. 67
|
| City |
Mainz |
| ZIP/Postal code |
55101 |
| Country |
Germany |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE14886 |
Expression data in HTETOP cells following tetracycline or dexrazoxane treatment |
|
| Relations |
| Reanalyzed by |
GSE119087 |
