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| Status |
Public on May 17, 2021 |
| Title |
P. falciparum heat shock response in a PfAP2-HS-defective population compared to its wild type control. |
| Organism |
Plasmodium falciparum |
| Experiment type |
Expression profiling by array
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| Summary |
Periodic fever is the most characteristic clinical feature of human malaria. However, how parasites survive malarial febrile episodes, which often involve temperatures of >40ºC, is not known. Plasmodium falciparum cultures adapted to periodic heat shock were used to identify PfAP2-HS as a transcription factor that is essential for survival at febrile temperatures. Transcriptomic analysis was performed to compare the effect of heat shock in parasites presenting the wild type form of PfAP2-HS (10E) and parasites that have defects in this protein, either a premature stop codon mutation (10G) or the deletion of the entire pfap2-hs gene (10E_pfap2-hs). A timecourse analysis including samples taken before, during and after heat shock revealed that the initial phase of the PfAP2-HS-dependent response is largely restricted to hsp70-1 and hsp90.
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| Overall design |
Time-course transcriptomic analysis of 10E, 10G and 10E_pfap2-hs cultures under control (35C) and heat shock conditions (HS, 41.5C for 3 h). Parasites were tightly sinchronized to a 5 h age window and HS was started at 30-35 hpi (33-38 hpi for the 10E_pfap2-hs line that develops more slowly). Samples for transcriptomic analysis were collected before (0 h), during (1.5 h and 3 h) and after HS (2 h post). The reference pool consisted of RNA from 3D7-A cultures, maintained under control conditions, throughout the asexual blood cycle. Total RNA was harvested using the Trizol method. A total number of 21 individual samples were analysed using the microarray design AMADID-084561 (GEO ID: GPL26985).
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| Contributor(s) |
Tintó-Font E, Michel-Todó L, Cortés A |
| Citation(s) |
34400833 |
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| Submission date |
Apr 27, 2020 |
| Last update date |
Sep 08, 2021 |
| Contact name |
Elisabet Tinto-Font |
| E-mail(s) |
elisabet.tinto@gmail.com
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| Organization name |
ISGlobal
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| Department |
Malaria
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| Street address |
c/Rossello 153
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| City |
Barcelona |
| ZIP/Postal code |
08036 |
| Country |
Spain |
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| Platforms (1) |
| GPL28456 |
Agilent-084561 P falciparum 15K Array |
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| Samples (21)
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| GSM4498902 |
10E 3h, Control |
| GSM4498903 |
10E 3h HS, HS-exposed |
| GSM4498904 |
10E 2h post, Control |
| GSM4498905 |
10E 2h post HS, HS-exposed |
| GSM4498906 |
10G Pre HS |
| GSM4498907 |
10G 1.5h, Control |
| GSM4498908 |
10G 1.5h HS, HS-exposed |
| GSM4498909 |
10G 3h, Control |
| GSM4498910 |
10G 3h HS, HS-exposed |
| GSM4498911 |
10G 2h post, Control |
| GSM4498912 |
10G 2h post HS, HS-exposed |
| GSM4498913 |
10E_pfap2-hs Pre HS |
| GSM4498914 |
10E_pfap2-hs 1.5h, Control |
| GSM4498915 |
10E_pfap2-hs 1.5h HS, HS-exposed |
| GSM4498916 |
10E_pfap2-hs 3h, Control |
| GSM4498917 |
10E_pfap2-hs 3h HS, HS-exposed |
| GSM4498918 |
10E_pfap2-hs 2h post, Control |
| GSM4498919 |
10E_pfap2-hs 2h post HS, HS-exposed |
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| This SubSeries is part of SuperSeries: |
| GSE149394 |
Transcriptomic analysis of heat shock resistance in Plasmodium falciparum |
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| Relations |
| BioProject |
PRJNA628606 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE149392_RAW.tar |
33.1 Mb |
(http)(custom) |
TAR (of TXT) |
| GSE149392_SupplementaryGEO_GeneLevel_Imputed_20200424.xls.gz |
1.2 Mb |
(ftp)(http) |
XLS |
| GSE149392_SupplementaryGEO_GeneLevel_Raw_20200424.xls.gz |
1.2 Mb |
(ftp)(http) |
XLS |
| Processed data included within Sample table |
| Processed data are available on Series record |
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