GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE149686 Query DataSets for GSE149686
Status Public on Feb 14, 2023
Title Warm temperature-triggered developmental reprogramming requires VIL1-mediated, genome-wide H3K27me3 accumulation in Arabidopsis
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary Through RNA-seq and ChIP-seq we show that VIL1 mediates warm ambient temperature responses.
Overall design We grew Col-0 (WT) and vil1-1 mutant at two different temperatures (22C and 27C) and compared the transcriptome. RNA-seq analysis shows that vil1 fails to downregulate high ambient temperature suppressed genes. H3K27me3 ChIP-seq showed high temperature-mediated accumulation of H3K27me3 compromised in vil1. We also found that vil1 suppresses constitutive thermo-morphogenic transcriptome of arp6. RNA-seq analysis revealed that constitutive heat responsive transcriptome of arp6 reverted back to the wild-type levels in arp6vil1. This observation suggests an antagonistic relationship between VIL1 and ARP6. H2A.Z ChIP-seq showed that unlike wild type, vil1 fails to evict H2A.Z at high ambient temperature from high temperature responsive genes.
Web link http://10.1242/dev.201343
Contributor(s) Kim J, Bordiya Y, Zhao B, Sung S
Citation(s) 36762655
Submission date May 01, 2020
Last update date May 16, 2023
Contact name Sibum Sung
Organization name University of Texas at Austin
Street address NMS 5.110, Stop A5000
City Austin
State/province TX
ZIP/Postal code 78712
Country USA
Platforms (1)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (42)
GSM4508971 Col-0 22°C RNA-seq (replicate 1)
GSM4508972 Col-0 22°C RNA-seq (replicate 2)
GSM4508973 Col-0 27°C RNA-seq (replicate 1)
BioProject PRJNA629681
SRA SRP259909

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource 45.7 Mb (ftp)(http) BW 58.3 Mb (ftp)(http) BW 45.4 Mb (ftp)(http) BW 49.8 Mb (ftp)(http) BW 48.7 Mb (ftp)(http) BW 48.3 Mb (ftp)(http) BW 57.0 Mb (ftp)(http) BW 57.8 Mb (ftp)(http) BW 57.7 Mb (ftp)(http) BW 58.1 Mb (ftp)(http) BW 49.2 Mb (ftp)(http) BW 48.5 Mb (ftp)(http) BW
GSE149686_RPKM_normalized_gene_count.xlsx 6.0 Mb (ftp)(http) XLSX 59.2 Mb (ftp)(http) BW 52.4 Mb (ftp)(http) BW 60.5 Mb (ftp)(http) BW 48.7 Mb (ftp)(http) BW 45.6 Mb (ftp)(http) BW 53.6 Mb (ftp)(http) BW 42.7 Mb (ftp)(http) BW 49.8 Mb (ftp)(http) BW
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap