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| Status |
Public on Apr 30, 2023 |
| Title |
Expression data from canine alveolar macrophages |
| Platform organism |
synthetic construct |
| Sample organism |
Canis lupus familiaris |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
MicroRNAs (miRNAs), a class of noncoding RNAs measuring 18 to 23 nucleotides (nt) that play important regulatory roles in host-virus interactions. Avian-origin H3N2 canine influenza virus (CIV) has emerged as the most prevalent subtype among dogs in Asia since 2007. To evaluate the roles of host miRNAs in H3N2 CIV infection, here, miRNA profiles obtained from primary canine bronchiolar epithelial cells (CBECs) and canine alveolar macrophages (CAMCs) were compared between infected and mock-infected cells with the H3N2 CIV JS/10. It was found that cfa-miR-125b, cfa-miR-151 and cfa-miR-423a expressions were significantly decreased in CIV-infected canine primary cells. Bioinformatics prediction indicated that 5’ seed regions of three miRNAs are partially complementary to the mRNAs of nucleoprotein (NP) and non-structural protein 1 (NS1) of JS/10. As determined by virus titration, quantitative real-time PCR (qRT-PCR) and western blotting, overexpression of cfa-miR-125b and cfa-miR-151 inhibited CIV infection, whereas overexpression or inhibition of cfa-miR-423a inhibited this infection. These results indicated that CIV replication could be regulated by miRNAs from host cells infected with CIV. Our findings support the notion that cellular miRNAs can inhibit virus infection, help to elucidate the resistance of host cells to viral infection and to clarify the pathogenesis of H3N2 CIV.
We used microarrays to detail the global programme of gene expression of primary canine alveolar macrophages (CAMCs) compared between infected and mock-infected cells with the H3N2 canine influenza virus (CIV) JS/10.
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| Overall design |
Total RNA was extracted from 1×10e6 cells of primary CAMCs infected and mock-infected with JS/10 for 24 h using TRIzol reagent. RNA samples were spectroscopically verified for purity, quality of the intact RNA without genomic DNA contamination was assessed using an Agilent 2100 Bioanalyzer. For infected and mock-infected CAMCs, an Affymetrix miRNA4.0 canine microRNA Microarray Kit (8×15 K) was used to profile the microRNA transcripts on the Affymetrix Technologies microRNA Platform. The probe in microarray chip is specifically designed for known canine miRNA sequences.
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| Contributor(s) |
Xie X, Pang M, Liu Y |
| Citation missing |
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| Submission date |
May 01, 2020 |
| Last update date |
May 02, 2023 |
| Contact name |
Maoda Pang |
| E-mail(s) |
pangmaoda@jaas.ac.cn
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| Organization name |
Nanjing Agricultural University
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| Street address |
NO.1 Zhongling Street
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| City |
Nanjing |
| ZIP/Postal code |
210000 |
| Country |
China |
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| Platforms (1) |
| GPL21572 |
[miRNA-4] Affymetrix Multispecies miRNA-4 Array [ProbeSet ID version] |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA629782 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE149700_RAW.tar |
2.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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