|
| Status |
Public on Apr 30, 2023 |
| Title |
Virus_infected cells, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Canine alveolar macrophage
|
| Organism |
Canis lupus familiaris |
| Characteristics |
breed: beagle
cell type: canine alveolar macrophages (CAMCs)
infection: H3N2 canine influenza virus (CIV) JS/10
|
| Treatment protocol |
Primary CAMCs were seeded onto sterile 24-well plates at a density of 2×105 cells/well, infected with JS/10 or phosphate buffered saline (PBS).
|
| Growth protocol |
Primary canine alveolar macrophages (CAMCs) were obtained from broncho-alveolar lavage (BAL) of beagle dog and cultured at 37 °C under 5% CO2 atmosphere.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from 1×10e6 cells of primary CAMCs infected and mock-infected with JS/10 for 24 h using TRIzol reagent (Invitrogen, New York, USA). RNA samples were spectroscopically verified for purity, quality of the intact RNA without genomic DNA contamination was assessed using an Agilent 2100 Bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Canine Genome 2.0 Array.
|
| Scan protocol |
GeneChips were scanned using an Affymetrix GCS 3000 7G Scanner.
|
| Description |
Expression data from canine alveolar macrophages
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
May 01, 2020 |
| Last update date |
Apr 30, 2023 |
| Contact name |
Maoda Pang |
| E-mail(s) |
pangmaoda@jaas.ac.cn
|
| Organization name |
Nanjing Agricultural University
|
| Street address |
NO.1 Zhongling Street
|
| City |
Nanjing |
| ZIP/Postal code |
210000 |
| Country |
China |
| |
|
| Platform ID |
GPL21572 |
| Series (1) |
| GSE149700 |
Expression data from canine alveolar macrophages |
|
