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Series GSE150790 Query DataSets for GSE150790
Status Public on May 19, 2020
Title Ribosome-quality control antagonizes the activation of integrated-stress response on colliding ribosomes
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by high throughput sequencing
Other
Summary Ribosome stalling occurring on aberrant mRNA activates quality control pathways to maintain proteostasis. Recently, ribosome stalling has also been linked to the activation of Gcn2 and the subsequent integrated-stress response (ISR). How the two processes are coordinated is not completely clear. Here we show that activation of ribosome-quality control by Hel2 suppresses that of Gcn2 in yeast. In the absence of Hel2, we observe a gene-expression signature indicative of ISR activation, suggesting that factor is used to suppress premature activation of Gcn2 in the absence of stress conditions. We further show that Hel2 and Gcn2 are activated by similar set of agents that cause ribosome stalling, with Hel2’s maximal activation occurring at lower frequency of stalling. Interestingly, inactivation of one pathway was found to result in the overactivation of the other, suggesting that both are activated by the same signal. Indeed, we provide evidence that suggests that, similar to Hel2, Gcn2 is activated by ribosome collisions. Collectively, our findings provide interesting details about how the multiple pathways that recognize stalled ribosomes coordinate to mount the appropriate response.
 
Overall design Libraries were constructed from RNA collected from various S. cerevisiae strains treated with or without methyl methanesulfonate (MMS).
 
Contributor(s) Zaher HS, Yan LL
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Submission date May 18, 2020
Last update date May 19, 2020
Contact name Liewei Leo Yan
Organization name Washington University in St Louis
Department Biology
Lab Zaher Lab
Street address 1 brookings dr.
City st louis
State/province MO
ZIP/Postal code 63139
Country USA
 
Platforms (1)
GPL27812 Illumina NovaSeq 6000 (Saccharomyces cerevisiae)
Samples (12)
GSM4558774 Sample 1_Wild-type; Biorep 1
GSM4558775 Sample 2_Wild-type;MMS treated;Biorep 1
GSM4558776 Sample 3_∆hel2; Biorep 1
Relations
BioProject PRJNA633588
SRA SRP262188

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE150790_processed_all.gene_counts.txt.gz 744.1 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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