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| Status |
Public on Dec 31, 2020 |
| Title |
Centromeric R loops contribute to defects in kinetochore assembly and chromosomal instability |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Other
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| Summary |
We mapped ssDNA formation in WT and hpr1delta cells at permissive (30C) and non-permissive (37C) temperature. We showed that hpr1 cells accumulate ssDNA show elevated levels of ssDNA at centromeric regions. Additionally, we show replication fork-associated ssDNA at origins of replication is more confined to the origins in hpr1 cells than WT cells, suggesting a defect in replication fork progression.
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| Overall design |
We queried the yeast genome for ssDNA production in WT and hpr1delta cells. G1-arrested cells were released into S phase at 30C and 37C and collected at 10, 15, 20, and 25 min. Each S phase sample was paired with a G1 control sample and subject to ssDNA labeling with Cy3 and Cy5, respectively, prior to co-hybridization to the same microarray. The level of ssDNA was quantified as the ratio of Cy3 signals to Cy5 signals for every probe position on the microarray, after normalization.
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| Contributor(s) |
Peng J, Chakraborty A, Feng W |
| Citation(s) |
33147102 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R00 GM081378 |
Single Stranded DNA: The Genome's Achilles Heel |
SUNY Upstate Medical University |
WENYI FENG |
| R01 GM118799 |
Mechanisms of replication stress-induced chromosome fragility |
SUNY Upstate Medical University |
WENYI FENG |
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| Submission date |
Jun 04, 2020 |
| Last update date |
Apr 01, 2021 |
| Contact name |
Wenyi Feng |
| E-mail(s) |
fengw@upstate.edu
|
| Phone |
3154648701
|
| Organization name |
SUNY Upstate Medical University
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| Department |
Biochemistry and Molecular Biology
|
| Lab |
Wenyi Feng
|
| Street address |
750 East Adams Street
|
| City |
Syracuse |
| State/province |
NY |
| ZIP/Postal code |
13210 |
| Country |
USA |
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| Platforms (1) |
| GPL10930 |
Agilent-014810 Yeast Whole Genome ChIP-on-Chip Microarray 4x44K (G4493A) [Probe Name version] |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA637395 |
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