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| Status |
Public on Dec 25, 2009 |
| Title |
Response of the Pseudomonas host chromosomal transcriptome to carriage of the IncP-7 plasmid pCAR1. |
| Platform organisms |
Pseudomonas; Pseudomonas putida KT2440; Pseudomonas fluorescens Pf0-1; Pseudomonas aeruginosa PAO1 |
| Sample organisms |
Pseudomonas putida KT2440; Pseudomonas fluorescens Pf0-1; Pseudomonas aeruginosa PAO1 |
| Experiment type |
Expression profiling by genome tiling array
|
| Summary |
Plasmid carriage requires appropriate expression of the genes on the plasmid or host chromosome through cooperative transcriptional regulation. To clarify the impact of plasmid carriage on the host chromosome, we compared the chromosomal RNA maps of plasmid-free and plasmid-containing host strains using the incompatibility group P-7 archetype plasmid pCAR1, which is involved in carbazole degradation, and three distinct Pseudomonas strains. The possession of pCAR1 altered gene expression related to the iron acquisition systems in each host. Expression of the major siderophore pyoverdine was greater in plasmid-containing P. putida KT2440 and P. aeruginosa PAO1 than in the plasmid-free host strains, in part due to the expression of carbazole-degradative genes on pCAR1. The mexEFoprN operon encoding an efflux pump of the resistance-nodulation-cell division (RND) family was specifically upregulated by the carriage of pCAR1 in P. putida KT2440, whereas the expression of orthologous genes in the other species remained unaltered. Induction of the mexEFoprN genes increased the resistance of pCAR1-containing KT2440 to chloramphenicol compared to pCAR1-free KT2440. Our findings indicate that the possession of pCAR1 altered the growth rate of the host via the expression of genes on pCAR1 and the host chromosomes.
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| Overall design |
Samples GSM380019-GSM380021: 6 samples were compared including two biological replicates.
Samples GSM380025-GSM380026: 4 samples were compared including two biological replicates.
Samples GSM380027-GSM380028: 4 samples were compared including two biological replicates.
Samples GSM380029-GSM380030: 4 samples were compared including two biological replicates.
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| Contributor(s) |
Shintani M, Takahashi Y, Tokumaru H, Kadota K, Hara H, Miyakoshi M, Naito K, Yamane H, Nishida H, Nojiri H |
| Citation(s) |
19930443 |
| |
| Submission date |
Mar 13, 2009 |
| Last update date |
Dec 10, 2013 |
| Contact name |
Hideaki Nojiri |
| E-mail(s) |
anojiri@mail.ecc.u-tokyo.ac.jp
|
| Phone |
+81-3-5841-3067
|
| Organization name |
The University of Tokyo
|
| Department |
Biotechnology Research Center
|
| Lab |
Laboratory of Environmental Biochemistry
|
| Street address |
1-1-1 Yayoi, Bunkyo-ku
|
| City |
Tokyo |
| ZIP/Postal code |
113-8657 |
| Country |
Japan |
| |
|
| Platforms (4)
|
| GPL6591 |
[pCAR1_8b520435F] Affymetrix CustomExpress IncP-7 plasmid pCAR1 Tiling Array |
| GPL8296 |
[KT2440b520511F] Pseudomonas putida KT2440 chromosome Array |
| GPL8297 |
Pseudomonas aeruginosa PAO1 chromosome array |
| GPL8298 |
Pseudomonas fluorescens Pf0-1 chromosome array |
|
| Samples (9)
|
| GSM380019 |
KT2440(pCAR1)_CustomExpress IncP-7 plasmid pCAR1 Tiling Array |
| GSM380020 |
PAO1(pCAR1)_CustomExpress IncP-7 plasmid pCAR1 Tiling Array |
| GSM380021 |
Pf0-1(pCAR1)_CustomExpress IncP-7 plasmid pCAR1 Tiling Array |
| GSM380025 |
KT2440_Pseudomonas putida KT2440 chromosome Array |
| GSM380026 |
KT2440(pCAR1)_Pseudomonas putida KT2440 chromosome Array |
| GSM380027 |
PAO1_Pseudomonas aeruginosa PAO1 chromosome array |
| GSM380028 |
PAO1(pCAR1)_Pseudomonas aeruginosa PAO1 chromosome array |
| GSM380029 |
Pf0-1_Pseudomonas fluorescens Pf0-1 chromosome array |
| GSM380030 |
Pf0-1(pCAR1)_Pseudomonas fluorescens Pf0-1 chromosome array |
|
| Relations |
| BioProject |
PRJNA116463 |
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