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| Status |
Public on Apr 27, 2021 |
| Title |
Systematic Analysis of Long Noncoding RNA and mRNA in Granulosa Cells During the Hen Ovulatory Cycle |
| Organism |
Gallus gallus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In this study, gene expression in Rohman layers was investigated at three distinct stages of the ovulatory cycle: zeitgeber time 0 (ZT0, 9:00 a.m.), zeitgeber time 12 (ZT12, 9:00 p.m.), and zeitgeber time 20 (ZT20, 5:00 a.m.) representing the early, middle, and LH surge stages, respectively, of the ovulatory cycle. Gene expression profiles were explored during follicle development at ZT0, ZT12, and ZT20 using Ribo-Zero RNA sequencing.
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| Overall design |
At 210-days, the birds were sacrificed at 9:00 AM (ZT0, N=3), 21:00 PM (ZT12, N=3) and 05:00 AM (ZT20, N=3). For total RNA sequencing, about 4 μg total RNA was isolated from the granulosa cells (a total of 9 samples).
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| Contributor(s) |
Li L, Hu S, Gui T, Deng X |
| Citation(s) |
34070248 |
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| Submission date |
Jun 11, 2020 |
| Last update date |
Jul 02, 2021 |
| Contact name |
Liang Li |
| Organization name |
Sichuan agricultural university
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| Street address |
Wenjiangqu huiminlu211hao sichuan nongyedaxue
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| City |
Chengdu |
| State/province |
Sichuan |
| ZIP/Postal code |
611130 |
| Country |
China |
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| Platforms (1) |
| GPL26853 |
Illumina NovaSeq 6000 (Gallus gallus) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA638841 |
| SRA |
SRP266920 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE152268_lnc_expression.txt.gz |
340.9 Kb |
(ftp)(http) |
TXT |
| GSE152268_mRNA_expression.txt.gz |
649.5 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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