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| Status |
Public on Jul 24, 2017 |
| Title |
Transcriptional analysis of Escherichia coli O157:H7 delta yhaO in MEM-HEPES |
| Organism |
Escherichia coli |
| Experiment type |
Expression profiling by array
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| Summary |
Deletion of yhaO was found to signifcantly decrease type three secretion in EHEC O157:H7. Transcriptional profiles of Escherichia coli O157: H7 and the isogenic yhaO mutant were generated and compared.
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| Overall design |
RNA was extracted from biological tripicate flasks and labelled with Cy3 or Cy5. Reference RNAs (w.t. TUV93-0) were pooled and divided into 80pmol aliquots for hybridisation. 80pmol of test Cy3 labelled cDNA was mixed with 80 pmol of pooled reference Cy5 labelled cDNA and hybrised to a single micorarray. Triplicate microarrays were scanned and data analysed using Genespring GX 7.3
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| Contributor(s) |
Tree JJ, Gally DL, Roe AJ |
| Citation missing |
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| |
| Submission date |
Mar 17, 2009 |
| Last update date |
Jul 24, 2017 |
| Contact name |
Jai Justin Tree |
| E-mail(s) |
j.tree@unsw.edu.au
|
| Phone |
+61 2 938 59142
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| Organization name |
University of New South Wales
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| Department |
School of Biotechnology and Biomolecular Sciences
|
| Lab |
Tree lab
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| Street address |
Rm s110 Bldg F25, UNSW, Gate 11 Botany St
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| City |
Sydney |
| State/province |
NSW |
| ZIP/Postal code |
2033 |
| Country |
Australia |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA129017 |
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