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| Status |
Public on Jun 28, 2020 |
| Title |
Optimized design of antisense oligomers for targeted rRNA depletion |
| Organisms |
Danio rerio; Xenopus laevis |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
RNA sequencing (RNA-seq) has become a standard method for quantifying gene expression transcriptome-wide. Due to the extremely high proportion of ribosomal RNA (rRNA) in total RNA, sequencing libraries usually incorporate messenger RNA (mRNA) enrichment. Although polyadenylate (poly(A)) tail selection is widely used, many applications require alternate approaches such as rRNA depletion. Recently, selective rRNA digestion, using RNaseH and antisense DNA oligomers that tile the length of target RNAs, has emerged as an easy, cost-effective alternative to commercial rRNA depletion kits. Here, we present a streamlined RNaseH-mediated rRNA depletion method that uses shorter antisense oligos that only sparsely tile the target RNA, in a digestion reaction of only 5 minutes. We wrote a Web tool, Oligo-ASST, that simplifies oligo design to favor target regions with optimal thermodynamic properties, and additionally allows users to design common oligo pools that can simultaneously target divergent RNAs in their regions of higher sequence similarity. We demonstrate the efficacy of these oligos by building rRNA-depleted sequencing libraries for Xenopus laevis as well as zebrafish, which expresses two distinct versions of the 28S, 18S, 5.8S, and 5S rRNAs during embryogenesis. These libraries efficiently deplete rRNA to <5% of total reads, on par with poly(A) selection, and also reveal expression of many non-adenylated RNA species. Oligo-ASST is freely available at https://mtleelab.pitt.edu/oligo to design antisense oligos for any taxon or to target any abundant RNA for depletion.
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| Overall design |
RNA-seq with different mRNA enrichment methods
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| Contributor(s) |
Lee MT, Phelps WA |
| Citation(s) |
33221877 |
| Submission date |
Jun 20, 2020 |
| Last update date |
Jun 27, 2022 |
| Contact name |
Miler T Lee |
| E-mail(s) |
miler@pitt.edu
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| Organization name |
University of Pittsburgh
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| Department |
Biological Sciences
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| Street address |
4249 Fifth Ave
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| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15260 |
| Country |
USA |
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| Platforms (2) |
| GPL20828 |
Illumina NextSeq 500 (Danio rerio) |
| GPL21248 |
Illumina NextSeq 500 (Xenopus laevis) |
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| Samples (13)
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| Relations |
| BioProject |
PRJNA640808 |
| SRA |
SRP268142 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE152902_xl_tpm.tsv.gz |
841.4 Kb |
(ftp)(http) |
TSV |
| GSE152902_zf_tpm.tsv.gz |
496.2 Kb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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