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Series GSE15352 Query DataSets for GSE15352
Status Public on Jan 01, 2010
Title Dynamic transcriptional and metabolic responses in yeast adapting to temperature stress.
Platform organisms Schizosaccharomyces pombe; Saccharomyces cerevisiae
Sample organism Saccharomyces cerevisiae
Experiment type Expression profiling by array
Summary Understanding the response processes in cellular systems to external perturbations is a central goal of large-scale molecular profiling experiments. We investigated the molecular response of yeast to increased and lowered temperatures relative to optimal reference conditions across two levels of molecular organization: the transcriptome using a whole yeast genome microarray and the metabolome applying the GC/MS technology with in-vivo stable-isotope labeling for accurate relative quantification of a total of 50 different metabolites. The molecular adaptation of yeast to increased or lowered temperatures relative control conditions at both the metabolic and transcriptional level is dominated by temperature-inverted differential regulation patterns of transcriptional and metabolite responses and the temporal response observed to be biphasic. The set of previously described general environmental stress response (ESR) genes showed particularly strong temperature-inverted response patterns. Among the metabolites measured, trehalose was detected to respond strongest to the temperature stress and with temperature-inverted up and downregulation relative to control, mid-temperature conditions. Although associated with the same principal environmental parameter, the two different temperature regimes caused very distinct molecular response patterns at both the metabolite and the transcript level. While pairwise correlations between different transcripts and between different metabolites were found generally preserved under the various conditions, substantial differences were also observed indicative of changed underlying network architectures or modified regulatory relationships. Gene and associated gene functions were identified that are differentially regulated specifically under the gradual stress induction applied here compared to abrupt stress exposure investigated in previous studies, including genes of as of yet unidentified function and genes involved in protein synthesis and energy metabolism.

Reference: Strassburg K, Walther D, Takahashi H, Kanaya S, and Kopka J. Dynamic transcriptional and metabolic responses in yeast adapting to temperature stress. OMICS JIB, 2010, 14(3), in press.
 
Overall design Time-series with 8 time points for three different ambient temperatures. Single replicate per time point and temperature series.
 
Contributor(s) Strassburg K, Kopka J, Walther D
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Submission date Mar 23, 2009
Last update date Feb 21, 2017
Contact name Dirk Walther
E-mail(s) walther@mpimp-golm.mpg.de
Phone 49-331-5678108
Organization name Max Planck Institute for Molecular Plant Physiology
Department Bioinformatics
Street address Am Mühlenberg 1
City Potsdam-Golm
ZIP/Postal code 14476
Country Germany
 
Platforms (1)
GPL2529 [Yeast_2] Affymetrix Yeast Genome 2.0 Array
Samples (24)
GSM385479 cold 10degC 0min
GSM385480 cold 10degC 15min
GSM385481 cold 10degC 30min
Relations
BioProject PRJNA117133

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE15352_RAW.tar 39.4 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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