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Series GSE153969 Query DataSets for GSE153969
Status Public on Jul 08, 2020
Title Control of Translation during the Unfolded Protein Response in Maize seedlings: Life without PERKs.
Organism Zea mays
Experiment type Expression profiling by high throughput sequencing
Other
Summary The accumulation of misfolded proteins in the endoplasmic reticulum (ER) defines a condition called ER stress that induces the unfolded protein response (UPR). The UPR in mammalian cells attenuates protein synthesis initiation, which prevents the piling up of misfolded proteins in the ER. Mammalian cells rely on Protein Kinase RNA-Like Endoplasmic Reticulum Kinase (PERK) phosphorylation of eIF2alpha to arrest protein synthesis, however, plants do not have a PERK homolog, so the question is whether plants control translation in response to ER stress. We compared changes in RNA levels in the transcriptome to the RNA levels protected by ribosomes and found a decline in translation efficiency, including many UPR genes, in response to ER stress. The decline in translation efficiency is due to the fact that many mRNAs are not loaded onto polyribosomes (polysomes) in proportion to their increase in total RNA, instead some of the transcripts accumulate in stress granules (SGs). The RNAs that populate SGs are not derived from the disassembly of polysomes because protein synthesis remains steady during stress. Thus, the surge in transcription of UPR genes in response to ER stress is accompanied by the formation of SGs, and the sequestration of mRNAs in SGs may serve to temporarily relieve the translation load during ER stress.
 
Overall design Examination of 0h, 6h and 12h tunicamycin treated maize seedling root samples with biological duplicates
 
Contributor(s) Kanodia P, Vijayapalani P, Srivastava R, Bi R, Liu P, Miller WA, Howell SH
Citation(s) 32743235
Submission date Jul 07, 2020
Last update date Aug 24, 2020
Contact name Wyatt Allen Miller
E-mail(s) wamiller@iastate.edu
Phone 5152942436
Organization name Iowa State University
Department Plant Pathology & Microbiology
Lab 4205 ATRB
Street address 2213 Pammel Dr
City Ames
State/province IA
ZIP/Postal code 50011
Country USA
 
Platforms (1)
GPL24631 Illumina HiSeq 3000 (Zea mays)
Samples (12)
GSM4661068 Riboseq_0hr_Rep1
GSM4661069 Riboseq_0hr_Rep2
GSM4661070 Riboseq_6hr_Rep1
Relations
BioProject PRJNA644623
SRA SRP270806

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Supplementary file Size Download File type/resource
GSE153969_CDS_mapped_count_data.xlsx 2.6 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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