Expression profiling by high throughput sequencing
Summary
This study evaluates the transcriptome of Arabidopsis thaliana seedlings exposed to the MAMP flg22 in the presence of 35 different bacteria
Overall design
Arabidopsis seeds (wild-type Col-0) were germinated on Johnson medium and grown for 7 days in the vertical position. Plants were then transferred to media containing 1 uM flg22 or not in the presence of a commensal bacteria strain (10E5 c.f.u / mL). Plants were grown in a growth chamber in a 15-h dark/9-h light regime (21°C day /18°C night). Whole seedlings were harvested twelve days after treatment. The experiment includes nine biological replicates from three independent experiments.
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