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Status |
Public on Aug 21, 2023 |
Title |
Next Generation Sequencing Facilitates Quantitative Analysis of Canine Cytotoxic T Cells Transcriptome |
Organism |
Canis lupus familiaris |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
This study aims to investigate the functions of cDLBCL-derived exosomes on cytotoxic T cells among peripheral blood mononuclear cells (PBMCs) in healthy dogs. Through RNA next-generation sequencing (NGS) and bioinformatics. Reveal the differences between the two groups with or without exosomes treatment. It includes the gene function list, protein profiles, the differentially expressed genes, the biological pathways, and the analysis of networks.
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Overall design |
Harvested canine CD8+ T cells to perform Transcriptome Next Generation Sequencing after co-culturing with 50 ug/ mL lymphoma exosomes for 48 hours. And vehicle controls were parallel treated with PBS. The results include two independent repeated experiments (N=2, N means the numbers of the samples from healthy dogs).
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Contributor(s) |
Lin C, Weng H |
Citation missing |
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Submission date |
Aug 21, 2020 |
Last update date |
Aug 21, 2023 |
Contact name |
Chen-Si Lin |
E-mail(s) |
cslin100@ntu.edu.tw
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Organization name |
Nationwide Taiwan University
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Department |
Department of Veterinary Medicine
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Lab |
Lab of Clinical Pathology and Cancer Research
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Street address |
No. 1, Sec. 4, Roosevelt Road
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City |
Taipei |
ZIP/Postal code |
10617 |
Country |
Taiwan |
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Platforms (1) |
GPL25760 |
Illumina NovaSeq 6000 (Canis lupus familiaris) |
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Samples (4)
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Relations |
BioProject |
PRJNA658600 |
SRA |
SRP278421 |