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| Status |
Public on Jan 05, 2011 |
| Title |
ChIP-on-Chip data from Rh30 cells +/- rapamycin treatment |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
p73 is a p53 family transcription factor that plays critical roles during development and tumor suppression. We analyzed p73 activity using a combination of ChIP-on-Chip and gene expression profiling, both at baseline and after treatment with the mTOR inhibitor rapamycin.
We report the first comprehensive analysis of p73 binding across the genome. Furthermore, we re-analyzed this p73 cistrome after perturbation with rapamycin, an inhibitor of mTOR and inducer of p73. Multiple determinants of p73 binding, activity, and function were evident, and were modulated by mTOR.
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| Overall design |
Rh30 rhabdomyosarcoma cells were treated with vehicle or 40 nM rapamycin for 24 h, then cross-linked and harvested.
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| Contributor(s) |
Rosenbluth JM, Pietenpol JA |
| Citation(s) |
21245298 |
| |
| Submission date |
Apr 16, 2009 |
| Last update date |
Mar 21, 2017 |
| Contact name |
Jennifer Pietenpol |
| Organization name |
Vanderbilt University Medical Center
|
| Department |
Biochemistry
|
| Lab |
652 PRB
|
| Street address |
23rd Ave South at Pierce
|
| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37232 |
| Country |
USA |
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| Platforms (7)
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| GPL4910 |
[Hs35b_P01R] Affymetrix Human Tiling 2.0R Set, Array 1 |
| GPL4911 |
[Hs35b_P02R] Affymetrix Human Tiling 2.0R Set, Array 2 |
| GPL4912 |
[Hs35b_P03R] Affymetrix Human Tiling 2.0R Set, Array 3 |
| GPL4913 |
[Hs35b_P04R] Affymetrix Human Tiling 2.0R Set, Array 4 |
| GPL4914 |
[Hs35b_P05R] Affymetrix Human Tiling 2.0R Set, Array 5 |
| GPL4915 |
[Hs35b_P06R] Affymetrix Human Tiling 2.0R Set, Array 6 |
| GPL4916 |
[Hs35b_P07R] Affymetrix Human Tiling 2.0R Set, Array 7 |
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| Samples (14)
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| This SubSeries is part of SuperSeries: |
| GSE15719 |
p73 activity and rapamycin treatment: ChIP-on-Chip and gene expression profiling studies |
|
| Relations |
| BioProject |
PRJNA123023 |
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