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| Status |
Public on Oct 10, 2020 |
| Title |
Three-dimensional human alveolar stem cell culture models reveal infection response to SARS-CoV-2 |
| Organism |
Chlorocebus aethiops |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2), which is the cause of a present global pandemic, infects human lung alveolar type 2 (hAT2) cells. Characterizing pathogenesis is crucial for developing vaccines and therapeutics. However, the lack of models mirroring the cellular physiology and pathology of hAT2 cells limits the study. Here, we develop a feeder-free, long-term three-dimensional (3D) culture technique for hAT2 cells, and investigate infection response to SARS-CoV-2. By imaging-based analysis and single-cell transcriptome profiling, we reveal rapid viral replication and the increased expression of interferon-associated genes and pro-inflammatory genes in infected hAT2 cells, indicating robust endogenous innate immune response. Further tracing of viral mutations acquired during transmission identifies full infection of individual cells effectively from a single viral entry. Our study provides deep insights into the pathogenesis of SARS-CoV-2, and the application of defined 3D hAT2 cultures as models for respiratory diseases.
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| Overall design |
Of total, six of SARS-CoV-2 infected Vero cells were deposited here. Two biological replicates were sequenced at post infection day 0, 1, and 2, respectively.
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| Contributor(s) |
Ju YS, Lee J, Choi B, Koh GY, Kim YT |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Oct 09, 2020 |
| Last update date |
Oct 12, 2020 |
| Contact name |
Young Seok Ju |
| E-mail(s) |
ysju@kaist.ac.kr
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| Organization name |
KAIST
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| Street address |
Daehak-ro 293
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| City |
Daejeon |
| ZIP/Postal code |
34141 |
| Country |
South Korea |
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| Platforms (1) |
| GPL22096 |
Illumina NextSeq 500 (Chlorocebus aethiops) |
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| Samples (6)
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| GSM4826501 |
SARS-CoV-2 infected Vero Cells at day 0 (control), replicate 1 |
| GSM4826502 |
SARS-CoV-2 infected Vero Cells at day 0 (control), replicate 2 |
| GSM4826503 |
SARS-CoV-2 infected Vero Cells at day 1, replicate 1 |
| GSM4826504 |
SARS-CoV-2 infected Vero Cells at day 1, replicate 2 |
| GSM4826505 |
SARS-CoV-2 infected Vero Cells at day 2, replicate 1 |
| GSM4826506 |
SARS-CoV-2 infected Vero Cells at day 2, replicate 2 |
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| Relations |
| BioProject |
PRJNA668354 |
| SRA |
SRP286895 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE159316_RAW.tar |
2.1 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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