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| Status |
Public on Aug 05, 2021 |
| Title |
Expression profiling of E. coli in three media treated with antibiotics |
| Organism |
Escherichia coli str. K-12 substr. MG1655 |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
RNA sequencing was performed on E. coli K12 MG1655 on three media (M9, CA-MHB, R10LB) treated with four antibiotics (Ciprofloxacin, Trimethoprim-sulfamethoxazole, Ceftriaxone, Meropenem) at their media-specific MIC90s
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| |
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| Overall design |
E. coli was grown to mid-log phase and treated with antibiotics for 30 min with shaking. Total RNA was sampled from independent duplicate cultures
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| |
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| Contributor(s) |
Sastry AV, Dillon N, Szubin R, Hefner Y, Xu S, Palsson BO |
| Citation(s) |
34431696 |
| |
| Submission date |
Oct 13, 2020 |
| Last update date |
Sep 14, 2021 |
| Contact name |
Anand Sastry |
| E-mail(s) |
avsastry@eng.ucsd.edu
|
| Organization name |
University of California, San Diego
|
| Department |
Bioengineering
|
| Lab |
Systems Biology Research Group
|
| Street address |
9500 Gilman Dr
|
| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21117 |
Illumina NextSeq 500 (Escherichia coli str. K-12 substr. MG1655) |
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| Samples (30)
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| Relations |
| BioProject |
PRJNA669053 |
| SRA |
SRP287299 |
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