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| Status |
Public on May 04, 2021 |
| Title |
The embryonic ontogeny of the gonadal somatic cells in mice and monkeys [sc3seq] |
| Organisms |
Macaca fascicularis; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In the early fetal stage, the gonads are bipotent and only later become the ovary or testis, depending on the genetic sex. Despite many studies examining how sex determination occurs from biopotential gonads, the spatial and temporal organization of bipotential gonads and their progenitors is poorly understood. Here, using lineage tracing in mice, we find that the gonads originate from a T+ primitive streak through WT1+ posterior intermediate mesoderm and appear to share origins anteriorly with the adrenal glands and posteriorly with the metanephric mesenchyme. Comparative single cell transcriptomic analyses in mouse and cynomolgus monkey embryos reveals the convergence of the lineage trajectory and genetic programs accompanying the specification of biopotential gonadal progenitor cells. This process involves sustained expression of epithelial genes and upregulation of mesenchymal genes, thereby conferring a unique epithelial/mesenchymal hybrid state. Our study provides key resources for understanding early gonadogenesis in mice and primates.
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| Overall design |
Single cell transcriptome analysis of mouse and cynomolgus monkey embryo using SC3-seq technology.
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| Contributor(s) |
Sasaki K, Oguchi A, Cheng K, Murakawa Y, Okamoto I, Ohta H, Yabuta Y, Iwatani C, Tsuchiya H, Yamamoto T, Seita Y, Saitou M |
| Citation(s) |
33951437 |
| |
| Submission date |
Oct 25, 2020 |
| Last update date |
May 17, 2021 |
| Contact name |
Yukihiro Yabuta |
| E-mail(s) |
yabyab@anat2.med.kyoto-u.ac.jp
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| Organization name |
Kyoto University, Graduate school of medicine
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| Department |
Anatomy and Cell Biology
|
| Street address |
Yoshida-Konoe-cho, Sakyo-ku
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| City |
Kyoto |
| State/province |
Kyoto |
| ZIP/Postal code |
606-8501 |
| Country |
Japan |
| |
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| Platforms (2) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL22523 |
Illumina NextSeq 500 (Macaca fascicularis) |
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| Samples (152)
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| This SubSeries is part of SuperSeries: |
| GSE160050 |
The embryonic ontogeny of the gonadal somatic cells in mice and monkeys |
|
| Relations |
| BioProject |
PRJNA671768 |
| SRA |
SRP288641 |
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