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| Status |
Public on Apr 01, 2010 |
| Title |
Effect of 1-methylcyclopropene (1-MCP) on preclimacteric nectarine (Prunus persica cv Stark Red Gold) fruit. |
| Organism |
Prunus persica |
| Experiment type |
Expression profiling by array
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| Summary |
Peach is a climacteric fruit whose ripening is largely dependent on ethylene. Recently, it has been shown that auxin is able to autonomously regulate the expression of ripening related genes, and that a cross-talk between ethylene and auxin occurs during the ripening process.
By using the non-destructing index of absorbance difference (IAD), mature nectarines were homogeneously grouped into three classes (0, 1 and 2) according to their ripening phase and treated with 1-methylcyclopropene (1-MCP), known to block ethylene receptors. 1-MCP responses differed in the three classes that were thus used to molecularly investigate the auxin-ethylene cross-talk during the transition from maturation to ripening and the accompanying switch of ethylene biosynthesis from System-1 to System-2. Microarray experiments showed that 1-MCP modified the expression of 121 genes (63 were up-regulated and 59 down-regulated). Besides inducing ethylene-, auxin- and ripening-repressed genes and repressing ethylene-, auxin- and ripening-induced genes, also genes induced by ripening, auxin and 1-MCP were discovered. The up-regulating effect of 1-MCP was observed for ctg134, similar to signalling peptides, for 1-aminocyclopropane-1-carboxylic acid synthase 1 (ACS1) and, interestingly, for many auxin-regulated genes, such as GH3, whose expression is widely used as a marker for free auxin. The latter finding, together with the induction of an IAA amidohydrolase, suggests that the 1-MCP application could have caused a rise in free auxin, thus leading to an increase in ethylene biosynthesis through the induction of the tightly regulated ACS1 gene.
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| Overall design |
A "direct comparison" approach has been used to investigate the effect at the transcriptional level of 1-methylcyclopropene (1-MCP) on preclimacteric nectarine (Prunus persica cv Stark Red Gold) fruit.
RNA has been extracted from either 1-MCP treated fruit or from fruit kept in air (control).
Four hybridization have been conducted for a total of four technical replicates (with dye-swap).
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| Contributor(s) |
Tadiello A, Trainotti L |
| Citation(s) |
26863869 |
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| Submission date |
May 26, 2009 |
| Last update date |
Jul 03, 2019 |
| Contact name |
Livio Trainotti |
| E-mail(s) |
livio.trainotti@unipd.it
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| Phone |
+390498276292
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| Organization name |
University of Padova
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| Department |
Biology
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| Lab |
Applied botany
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| Street address |
Via Ugo Bassi 58/B
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| City |
Padova |
| ZIP/Postal code |
35131 |
| Country |
Italy |
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| Platforms (1) |
| GPL8584 |
Prunus persica 5.7K microPEACH1.0 |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA117169 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE16224_RAW.tar |
1.1 Mb |
(http)(custom) |
TAR (of MEV) |
| Processed data included within Sample table |
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