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| Status |
Public on Aug 03, 2022 |
| Title |
Dimorphic H3K27 methylation guides germline or vegetative cell fate dichotomy in male gametophytes [ATAC-seq] |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
During angiosperm male gametogenesis, microspores divide to produce a vegetative cell (VC) and a male germline (MG), each with a distinct cell fate. How the MG cell/VC fate is determined remains largely unknown. Here, we report that the VC-targeted H3K27me3 erasure resulted in VC fate transition towards a gamete destination. Multi-omics and cytologic analysis reveal that H3K27me3 is essential for VC fate commitment and contributes to suppress the MG cell fate initiation in VC, whereas MG cells require H3K27me3 reprograming for the gamete cell fate. This work suggests that the MG cell/VC fate is epigenetically regulated. The dimorphic H3K27 methylation acts as a core switch to determine their distinct cell fates and ensure the functional specification of both VC and MG for pollen fertility. This work also provides direct evidences for the proposal that VC maintains the default developmental program of microspore, whereas MG requires reprogramming.
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| Overall design |
Examination of H3K27me3 modification (CUT&Tag), chromatin accessibility (ATAC-seq) and transcriptome (RNA-seq). This dataset includes the ATAC-seq data.
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| Contributor(s) |
Huang X, Sun M |
| Citation(s) |
35543471 |
| Submission date |
Dec 03, 2020 |
| Last update date |
Aug 04, 2022 |
| Contact name |
Xiaorong Huang |
| E-mail(s) |
huangxr@whu.edu.cn
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| Organization name |
College of Life Science, Wuhan university
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| Street address |
Bayi Road
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| City |
Wuhan |
| ZIP/Postal code |
430072 |
| Country |
China |
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| Platforms (1) |
| GPL26208 |
Illumina NovaSeq 6000 (Arabidopsis thaliana) |
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| Samples (10)
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| GSM4956109 |
V-Mt SC, ATAC, rep2 |
| GSM4956110 |
WT VC, ATAC, rep1 |
| GSM4956111 |
WT VC, ATAC, rep2 |
| GSM4956112 |
WT SC, ATAC, rep1 |
| GSM4956113 |
WT SC, ATAC, rep2 |
| GSM5975233 |
V-Mt VC, ATAC, rep3 |
| GSM5975234 |
V-Mt VC, ATAC, rep4 |
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| This SubSeries is part of SuperSeries: |
| GSE162640 |
Dimorphic H3K27 methylation guides germline or vegetative cell fate dichotomy in male gametophytes |
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| Relations |
| BioProject |
PRJNA682444 |
| SRA |
SRP295736 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE162638_RAW.tar |
1.7 Gb |
(http)(custom) |
TAR (of BW) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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