|
| Status |
Public on Mar 04, 2021 |
| Title |
single cell RNA sequencing on minipig PBMCs |
| Organism |
Sus scrofa domesticus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Single cell RNA sequencing was used to explore the long-term maintenance of immune memory cells and their functionality for the first time in this animal model. It revealed an increase in pertussis-specific T cells frequency in DTaP primed minipigs, together with up-regulation of genes involved in antigen presentation and interferon pathways.
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| |
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| Overall design |
6 samples were analyzed including 2 PBMC samples from control minipig, 2 PBMC samples from DTaP/Tdap immunized minipig incubated in vitro with medium, and 2 PBMC samples from DTaP/Tdap immunized minipig incubated in vitro with pertussis antigens pool.
|
| Web link |
https://www.frontiersin.org/articles/10.3389/fimmu.2021.613810/abstract
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| |
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| Contributor(s) |
Dégletagne C, Chautard E, Vaure C, Husson S, Courtois V |
| Citation(s) |
33815369 |
| |
| Submission date |
Jan 05, 2021 |
| Last update date |
Apr 07, 2021 |
| Contact name |
Emilie Chautard |
| E-mail(s) |
emilie.chautard@sanofi.com
|
| Organization name |
Sanofi
|
| Street address |
1541 Avenue Marcel Mérieux
|
| City |
Marcy L'Etoile |
| ZIP/Postal code |
69280 |
| Country |
France |
| |
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| Platforms (1) |
| GPL29562 |
Illumina NovaSeq 6000 (Sus scrofa domesticus) |
|
| Samples (6)
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| Relations |
| BioProject |
PRJNA689738 |
| SRA |
SRP300410 |
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