|
| Status |
Public on Mar 04, 2021 |
| Title |
TBS_ctrl_5 |
| Sample type |
SRA |
| |
|
| Source name |
PBMC
|
| Organism |
Sus scrofa domesticus |
| Characteristics |
strain: Gottingen
tissue: blood
age: post natal month 12
treatment: 3 TBS injections
|
| Treatment protocol |
PBMC extraction using ficoll gradient followed by 16h in vitro incubation with RPMI medium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cell suspension was mixed with master mix and loaded with Single Cell 3′ Gel Beads and Partitioning Oil into a Single Cell 3′ Chip. RNA transcripts from single cells were uniquely barcoded and reverse-transcribed within droplets. cDNA molecules were pooled and the cDNA pool then go through an end repair process, the addition of a single ‘A’ base, and then ligation of the adapters. The products are then purified and enriched with PCR to create the final cDNA library.
10x Genomics Chromium 3' v3 Single Cell Gene Expression Kit
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
16h in vitro incubation with RPMI medium
|
| Data processing |
Cellranger mkfastq v3.0.2 to generate FASTQ
Cell Ranger count v3.0.2 to generate 10X filtered output files
Genome_build: Sscrofa11.1, Ensembl release 98
Supplementary_files_format_and_content: [Cellranger files]
|
| |
|
| Submission date |
Jan 05, 2021 |
| Last update date |
Mar 05, 2021 |
| Contact name |
Emilie Chautard |
| E-mail(s) |
emilie.chautard@sanofi.com
|
| Organization name |
Sanofi
|
| Street address |
1541 Avenue Marcel Mérieux
|
| City |
Marcy L'Etoile |
| ZIP/Postal code |
69280 |
| Country |
France |
| |
|
| Platform ID |
GPL29562 |
| Series (1) |
| GSE164263 |
single cell RNA sequencing on minipig PBMCs |
|
| Relations |
| BioSample |
SAMN17215549 |
| SRA |
SRX9782930 |
