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| Status |
Public on Mar 05, 2021 |
| Title |
Chromatin accessibilty analysis of HCMV infected and mock-infected Kasumi-3 cells at 24 hours post infection |
| Organisms |
Homo sapiens; Human betaherpesvirus 5 |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Purpose: to compare host gene expression in HCMV infected and mock infected Kasumi-3 cells at 24 hours post infection and analyze viral chromatin accessibility
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| Overall design |
Kasumi-3 cells were infected with the TB40/E wtGFP strain of HCMV. At 4 hr post-infection, virus was inactivated and cells were kept in culture for additional 20 hours. Infected cells were sorted for GFP expression and live cells while mock infected cells for live cells. Sorted cells were collected and lysed in transposase reaction buffer as described below.
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| Contributor(s) |
Hummel M, Forte E, Schipma M |
| Citation(s) |
33731453 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| P01 AI112522 |
Integrating Mechanistic Insights from Diverse Models to Prevent CMV Reactivation following Transplantation |
NORTHWESTERN UNIVERSITY AT CHICAGO |
MARY A HUMMEL |
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| |
| Submission date |
Jan 14, 2021 |
| Last update date |
Jun 04, 2021 |
| Contact name |
Mary Hummel |
| E-mail(s) |
m-hummel@northwestern.edu
|
| Phone |
847-322-6438
|
| Organization name |
Northwestern University
|
| Street address |
303 E. Chicago Ave
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60611 |
| Country |
USA |
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| Platforms (2) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL24958 |
Illumina NextSeq 500 (Homo sapiens; Human betaherpesvirus 5) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE164861 |
Epigenetic reprogramming of host and viral genes by Human Cytomegalovirus infection in Kasumi-3 myeloid progenitor cells at early times post-infection |
|
| Relations |
| BioProject |
PRJNA692193 |
| SRA |
SRP301766 |
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