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| Status |
Public on Jan 01, 2023 |
| Title |
Effect of Insulin receptor deletion in hepatocytes on liver gene expression at three distinct time points in male mice (C57BL/6J) fed ad libitum a chow diet |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To assess the consequences of IR deletion on gene expression using microarray and its contribution of IR to the control of nocturnal and diurnal liver activity, we analyzed liver gene expression during the day (ZT8), at day-night transition (ZT12) and at night (ZT16).
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| Overall design |
To generate tamoxifen-inducible hepatocyte-specific IR knockout mouse line (IRhep-/-) and their control (IRhep+/+), animals carrying LoxP sites flanking the fourth exon of the IR gene (IRlox/lox stock number: 006955; Jackson Laboratory, Bar Harbor, ME, USA) were intercrossed with C57BL/6J mice, which specifically express Cre recombinase in the liver under the transthyretin promoter (TTR-CreTam mice), as previously described (Nemazanyy et al., 2015; Smati et al., 2020). Total liver RNA was extracted using TRI Reagent (Molecular Research Center, Inc., Cincinnati, OH, USA). Gene expression profiles were obtained at the GeT‐TRiX facility (GenoToul, Genopole Toulouse Midi-Pyrénées) using Agilent Sureprint G3 Mouse GE v2 microarrays (8x60K, design 074809) following the manufacturer's instructions.
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| Contributor(s) |
Fougeray T, Polizzi A, Régnier M, Fougerat A, Burnol A, Postic C, Montagner A, Guillou H, Naylies C, Lippi Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jan 20, 2021 |
| Last update date |
Jan 03, 2023 |
| Contact name |
Hervé Guillou |
| E-mail(s) |
herve.guillou@toulouse.inra.fr
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| Phone |
0582066389
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| Organization name |
INRAE
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| Department |
ToxAlim
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| Lab |
TIM
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| Street address |
180 Chemin de Tournefeuille
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| City |
Toulouse |
| ZIP/Postal code |
31027 |
| Country |
France |
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| Platforms (1) |
| GPL21163 |
Agilent-074809 SurePrint G3 Mouse GE v2 8x60K Microarray [Probe Name version] |
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| Samples (36)
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| This SubSeries is part of SuperSeries: |
| GSE165156 |
The hepatocyte insulin receptor is required to program rhythmic gene expression and the liver clock |
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| Relations |
| BioProject |
PRJNA693442 |
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