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| Status |
Public on Sep 15, 2022 |
| Title |
Effect of beta3 adrenergic activation on liver gene expression profile in mice (C57Bl/6J) expressing hepatocyte PPARalpha or not |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Pparα hepatocyte-specific knockout (Pparαhep-/-) mice were generated at INRAE’s rodent facility (Toulouse, France) by mating the floxed-Pparα mouse strain with C57BL/6J albumin-Cre transgenic mice to obtain albumin-Cre+/-Pparαflox/flox mice (LKO). Albumin-Cre-/-Pparαflox/flox (Pparαhep+/+) littermates were used as controls (LWT). Twelve week-old mice were transferred in a ventilated cabinet at the specific temperature of 30°C (thermoneutrality) 2 weeks before experiments. Mice were fed ad libitum or fasted at ZT0 and given beta3 adrenergic receptor agonist: CL316243 ((3 mg/kg body weight; Sigma Aldrich) = Beta3) or vehicle (0.5% carboxymethyl cellulose in sterilized water)=vehicle) by gavage at ZT10 and sacrificed at ZT16.
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| Overall design |
Gene expression profile was analyzed in 6 mice of each condition in both genotypes.
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| Contributor(s) |
Fougerat A, Polizzi A, Guillou H, Naylies C, Lippi Y, Soubès F |
| Citation(s) |
35675775 |
| Submission date |
Jan 26, 2021 |
| Last update date |
Sep 16, 2022 |
| Contact name |
Hervé Guillou |
| E-mail(s) |
herve.guillou@toulouse.inra.fr
|
| Phone |
0582066389
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| Organization name |
INRAE
|
| Department |
ToxAlim
|
| Lab |
TIM
|
| Street address |
180 Chemin de Tournefeuille
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| City |
Toulouse |
| ZIP/Postal code |
31027 |
| Country |
France |
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| Platforms (1) |
| GPL21163 |
Agilent-074809 SurePrint G3 Mouse GE v2 8x60K Microarray [Probe Name version] |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA694976 |
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