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| Status |
Public on Jun 03, 2010 |
| Title |
Genome-wide transcriptome analysis of two maize inbred lines under drought stress during the seedling stage |
| Organism |
Zea mays |
| Experiment type |
Expression profiling by array
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| Summary |
To understand the transcriptome changes during drought tolerance in maize, the drought-tolerant line Han21 and drought-sensitive line Ye478, which show substantial differences in drought tolerance at the seedling stage, were selected for this study. Using the GeneChip Maize Genome Arrays, we applied genome-wide gene expression analysis to the two genotypes under gradual drought stress and re-watering. We identified 2172 common regulated transcripts in both lines under drought stress, with 1084 common up-regulated transcripts and 1088 common down-regulated transcripts. Among the 2172 transcripts, 58 potential protein kinases and 117 potential transcription factors were identified. The potential components of the ABA signaling pathway were identified from the common regulated transcripts. We also identified 940 differentially regulated transcripts between the two lines. Among the 940 transcripts, the differential expression levels of 29 transporters and 15 cell wall-related transcripts may contribute to the different tolerances of the two lines. Additionally, we found that the drought-responsive genes in the tolerant Han21 line recovered more quickly when the seedlings were re-watered, and 311 transcripts in the tolerant Han21 line were exclusively up-regulated at the re-watering stage compared to the control and stress conditions. Our study provides a global characterization of two maize inbred lines during drought stress and re-watering and will be valuable for further study of the molecular mechanisms of drought tolerance in maize.
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| Overall design |
In two independent experiments, we generate maize gene expression profiles during drought stress and re-watering through comparing genome-wide expression patterns of drought stress treatment and re-watering treatment by using 17,555 Affymetrix maize whole genome array.
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| Contributor(s) |
Zheng J, Fu J, Gou M, Huai J, Liu Y, Jian M, Huang Q, Guo X, Dong Z, Wang H, Wang G |
| Citation(s) |
19953304 |
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| Submission date |
Jun 12, 2009 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Guoying Wang |
| E-mail(s) |
gywang@caas.net.cn
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| Phone |
86-10-62162927
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| Organization name |
Chinese Academy of Agricultural Sciences
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| Department |
Institute of Crop Sciences
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| Lab |
Maize Molecular Biology
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| Street address |
Zhongguancun Nandajie 12
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| City |
Beijing |
| ZIP/Postal code |
100081 |
| Country |
China |
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| Platforms (1) |
| GPL4032 |
[Maize] Affymetrix Maize Genome Array |
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| Samples (24)
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| GSM415939 |
Han21 moderate drought, biological rep2 |
| GSM415940 |
Han21 severe drought, biological rep1 |
| GSM415941 |
Han21 severe drought, biological rep2 |
| GSM415942 |
Han21 re-watering, biological rep1 |
| GSM415943 |
Han21 re-watering, biological rep2 |
| GSM415944 |
Ye478 control, biological rep1 |
| GSM415945 |
Ye478 control, biological rep2 |
| GSM415946 |
Ye478 moderate drought, biological rep1 |
| GSM415947 |
Ye478 moderate drought, biological rep2 |
| GSM415948 |
Ye478 severe drought, biological rep1 |
| GSM415949 |
Ye478 severe drought, biological rep2 |
| GSM415950 |
Ye478 re-watering, biological rep1 |
| GSM415951 |
Ye478 re-watering, biological rep2 |
| GSM416646 |
Han21 control, biological rep3 |
| GSM416647 |
Han21 moderate drought, biological rep3 |
| GSM416648 |
Han21 severe drought, biological rep3 |
| GSM416649 |
Han21 re-watering, biological rep3 |
| GSM416650 |
Ye478 control, biological rep3 |
| GSM416651 |
Ye478 moderate drought, biological rep3 |
| GSM416652 |
Ye478 severe drought, biological rep3 |
| GSM416653 |
Ye478 re-watering, biological rep3 |
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| Relations |
| BioProject |
PRJNA116209 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE16567_RAW.tar |
54.5 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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