|
| Status |
Public on Jun 03, 2010 |
| Title |
Han21 severe drought, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
The shoot tissue of the seedlings
|
| Organism |
Zea mays |
| Characteristics |
tissue: shoot
developmental stage: three-leaf stage seedlings
genotype: Han21
protocol: severe drought stress
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The total RNA was extracted by the TRIzol method (Invitrogen, USA). Mesocotyls were ground in liquid nitrogen, and 50-100 mg of frozen tissue was transferred into a tube with 1 ml TRIzol. Next, RNA was isolated, dried and dissolved in 50 µl DEPC-H2O. RNA quality and concentration were determined by 1.0% agarose gel electrophoresis and spectrophotometry.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 µg of cRNA were hybridized for 16 hr at 45℃ on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from maize inbred line Han21 severe drought stress
|
| Data processing |
The data were analyzed with GCOS software (Microarray Suite version 5.0, MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Jun 12, 2009 |
| Last update date |
Jun 03, 2010 |
| Contact name |
Guoying Wang |
| E-mail(s) |
gywang@caas.net.cn
|
| Phone |
86-10-62162927
|
| Organization name |
Chinese Academy of Agricultural Sciences
|
| Department |
Institute of Crop Sciences
|
| Lab |
Maize Molecular Biology
|
| Street address |
Zhongguancun Nandajie 12
|
| City |
Beijing |
| ZIP/Postal code |
100081 |
| Country |
China |
| |
|
| Platform ID |
GPL4032 |
| Series (1) |
| GSE16567 |
Genome-wide transcriptome analysis of two maize inbred lines under drought stress during the seedling stage |
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