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| Status |
Public on Nov 02, 2022 |
| Title |
Transcriptome analysis of gene expression divergence in BBX11 mutation in red light |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
phytochrome B (phyB) acts as the red light photoreceptor and negatively regulates the growth-promoting factor PHYTOCHROME INTERACTING 4 (PIF4) through a direct physical interaction, which in turn changes the expression of a large number of genes. phyB-PIF4 module regulates a variety of biological and developmental processes in plants. In this study, we demonstrate that B-BOX PROTEIN 11 (BBX11) physically interacts with both phyB and PIF4. BBX11 negatively regulates PIF4 accumulation as well as its biochemical activity, consequently leading to the repression of PIF4-controlled genes' expression and promotion of photomorphogenesis in the prolonged red light. This study reveals a regulatory mechanism that mediates red light signal transduction and sheds a light on phyB-PIF4 module in promoting red light-dependent photomorphognenesis.
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| Overall design |
Col-0, bbx11-1 and pif4-2 mutant seedlings were grown in constant red (94 μmol/m2/s) light for 4 d. The samples were harvested and used to isolate total RNA with an RNeasy Plant Mini Kit (Qiagen).
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| Contributor(s) |
Wang X, Xu D |
| Citation(s) |
36304757 |
| Submission date |
Feb 03, 2021 |
| Last update date |
Nov 03, 2022 |
| Contact name |
Xuncheng Wang |
| E-mail(s) |
1706380435@pku.edu.cn
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| Organization name |
Peiking University
|
| Street address |
5 Yiheyuan Road
|
| City |
Beijing |
| ZIP/Postal code |
100871 |
| Country |
China |
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| Platforms (1) |
| GPL26208 |
Illumina NovaSeq 6000 (Arabidopsis thaliana) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA699276 |
| SRA |
SRP304566 |
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