|
| Status |
Public on Mar 11, 2021 |
| Title |
Transcriptome profile of the anterior pituitary gland in pigs during maternal recognition of pregnancy |
| Platform organism |
Sus scrofa |
| Sample organism |
Sus scrofa domesticus |
| Experiment type |
Expression profiling by array
|
| Summary |
The knowledge regarding the reproductive-status-related changes occurring within the transcriptome of the porcine pituitary is limited. This study aimed to compare pituitary gland gene expression profiles of pigs during the maternal recognition of pregnancy period (days 12-13 of pregnancy) with pigs during the respective days of the estrous cycle using a microarray approach. Analysis indicated 482 differentially expressed genes (DEGs) with a FC ≥ 1.5 (p < 0.05) in the pituitary of pregnant vs. estrous-cyclic pigs. Among them, 68 were up-regulated and 414 were down-regulated. The evaluated DEGs were annotated to 39 gene ontology (GO) biological processes terms, 13 GO cellular components terms, and 10 GO molecular function terms. Among the evaluated DEGs were selected genes coding for proteins potentially involved in the regulation of early pregnancy in pigs and used for gene interaction analysis and validation of microarray results. Analysis of the relationships among DEGs during maternal recognition of pregnancy showed that some of them are connected with, i.e., TGFβ signaling pathway, follicle-stimulating hormone, prolactin, and growth hormone synthesis or activity, adipocytokines responsivity, angiogenesis, immune response, prostaglandin synthesis and inter-pituitary cell to cell interactions. The findings expand the knowledge regarding the molecular mechanisms appearing in the pituitary in pigs during the maternal recognition period of pregnancy.
|
| |
|
| Overall design |
We analized anterior pituitary glands obtained from 8 indyviduals (n=8). Pituitaries were collected from mature gilts on days 12-13 of early pregnancy (n = 4) and the estrous cycle (n = 4).
|
| |
|
| Contributor(s) |
Zmijewska A, Czelejewska W, Drzewiecka EM, Franczak A |
| Citation(s) |
36542881 |
| |
| Submission date |
Mar 10, 2021 |
| Last update date |
Jan 22, 2023 |
| Contact name |
Agata Zmijewska |
| E-mail(s) |
agata.zmijewska@uwm.edu.pl
|
| Phone |
+48 89 5233201
|
| Organization name |
Univeristy of Warmia and Mazury
|
| Department |
Demartment of Animal Anatomy and Physiology
|
| Street address |
Oczapowski 1a
|
| City |
Olsztyn |
| ZIP/Postal code |
10-719 |
| Country |
Poland |
| |
|
| Platforms (1) |
| GPL16571 |
Agilent-026440 Sus scrofa (Pig) Oligo Microarray v2 (Probe Name version) |
|
| Samples (4)
|
| GSM5151330 |
pregnant (Days 12-13) vs. cyclic (Days 12-13 of the estrous cycle) 1_1 |
| GSM5151331 |
pregnant (Days 12-13) vs. cyclic (Days 12-13 of the estrous cycle) 1_2 |
| GSM5151332 |
pregnant (Days 12-13) vs. cyclic (Days 12-13 of the estrous cycle) 1_3 |
| GSM5151333 |
pregnant (Days 12-13) vs. cyclic (Days 12-13 of the estrous cycle) 1_4 |
|
| Relations |
| BioProject |
PRJNA713018 |
Less...
More...