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Series GSE168709 Query DataSets for GSE168709
Status Public on Mar 11, 2024
Title mRNA expression data of Mes vs. Epi cells in mouse non-NE SCLC
Organism Mus musculus
Experiment type Expression profiling by array
Summary Distinct mRNA populatins have been detected in Epi vs. Mes cells of mouse non-NE SCLC
The aim of the study was to globally profile the differential gene expression in Mes vs. Epi cells of mouse non-NE SCLC.
Overall design Single clones from mouse non-NE SCLC were picked and expaned to obtain enough cells for further study. Total RNA was extracted and Labeled cRNA were purified by RNeasy mini kit (QIAGEN). Each slide was hybridized with 1.65ug Cy3-labeled cRNA using Gene Expression Kit (Agilent technologies, Santa Clara, CA, US), according to the manufacturer’s instructions. After 17 hours hybridization, slides were washed in staining dishes (Thermo Shandon, Waltham, MA, US) with Gene Expression Wash Buffer Kit (Agilent technologies, Santa Clara, CA, US) following the manufacturer’s instructions. Slides were scanned by Agilent Microarray Scanner (Agilent technologies, Santa Clara, CA, US) with default settings, Dye channel: Green, Scan resolution = 5 um, PMT 100%, 16 bits. Data were extracted with Feature Extraction software 10.7 (Agilent technologies, Santa Clara, CA, US). Raw data were normalized by Quantile algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US). GSEA was performed following the developer’s protocol (
Contributor(s) Ji H, Jin Y
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Submission date Mar 11, 2021
Last update date Mar 11, 2024
Contact name yujuan jin
Organization name Shanghai Institutes for Biological Sciences, CAS
Lab Hongbin Ji
Street address Yueyang Road 320#
City Shanghai
ZIP/Postal code 200031
Country China
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (10)
GSM5159488 Mes1
GSM5159489 Mes2
GSM5159490 Mes3
BioProject PRJNA713760

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE168709_BH12233-3_nor_data.txt.gz 6.0 Mb (ftp)(http) TXT
GSE168709_RAW.tar 21.3 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
Processed data are available on Series record

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